ZnCl[sub 2] and AlAl were administered either alone (Zn-and AlAl-treated groups) or in combination (AlAl + Zn-treated group). Blood and liver samples were collected 5 hours post treatment in all groups. Zinc was used because of its potential intracellular regulatory effect as a calcium ...
LC, GCLC, vitamin D, HOMA insulin resistance and cell viability assays GSH and LC levels were determined using high-performance liquid chromatography.14 The level of GCLC total protein in the plasma was determined using an ELISA kit (catalog #MBS 704124, MyBiosource, San Diego, CA, USA)....
Compared with the Sham group, the MI/R group had an increased myocardial infarction area (Fig. 1A). Myocardial injury was assessed by H&E staining, and there was more severe myocardial injury in the MI/R group than in the Sham group (Fig. 1B). Besides, ELISA elicited that the ...
Molecular docking experiment All docking experiments were performed using the Discovery Studio 2.5 software package (Accelrys Software Inc, San Diego, CA, USA). The protocol was generated using the CDOCKER protocol without the crystal waters. Before docking, hydrogen atoms were added to the unoccupied...
Following the digestion of the synovial tissue into a single-cell suspension, the cell suspension was incubated in the dark for 20 min with anti-CD86 (200305, BioLegend, San Diego, CA, USA). A pre-cooled staining buffer was used to remove unbound antibodies. The cells were fixed and perme...
The statistical calculations of the data were carried out by Prism 6.0 software (GraphPad Software Inc., San Diego, CA, USA) and all data were expressed as mean ± standard derivations (SD). Multigroup analyses were made by one-way analysis of variance (ANOVA) followed by a Student...
The data were assessed using Instat software (GraphPad Prism4, San Diego, CA, USA). The Student's t-test or one-way analysis of variance (ANOVA) was used for parametric data. Statistical significance was defined as p<0.05. Results
All statistical analyses were performed using GraphPad Prism 7.0 (GraphPad Software Inc., San Diego, CA, USA). The data were represented as mean ± standard deviation. Means between two groups were compared using Student’st-test, whereas those between multiple groups were compared using one/two...
All statistical analyses were performed using GraphPad Prism 7.0 (GraphPad Software Inc., San Diego, CA, USA). The data were represented as mean ± standard deviation. Means between two groups were compared using Student’s t-test, whereas those between multiple groups were compared using one/tw...
cells only in the retina of mice with hypoglycaemia, and not in the mice present in the euglycaemic group (Figure 3B); the two different retina isolated from hypoglycaemic (Hypo1 and Hypo2) and euglycaemic mice (Eugly1 and Eugly2) are representative of the four retina from each group. ...