GSDME (ab215191), caspase-3 (ab13847), caspase-1 (ab179515), pro-IL-1β (ab2105) and mature (m)IL-1β (ab9722) antibodies were obtained from Abcam (Cambridge, UK); cleaved caspase-1 (3866), cleaved caspase-3(9661) antibodies were obtained from Cell Signaling Technology, Inc. (Danv...
Antibody to GSDME (ab215191) was purchased from Abcam. Caspase-3 antibody (9662S), cleaved caspase-3 (9664S), caspase-9 antibody (9502S), and horseradish peroxidase-labeled rabbit secondary antibody (7074S) were purchased from Cell Signaling Technology (CST). β-acin (TA811000) was purchas...
GSDME (ab215191), caspase-3 (ab13847), caspase-1 (ab179515), pro-IL-1β (ab2105) and mature (m)IL-1β (ab9722) antibodies were obtained from Abcam (Cambridge, UK); cleaved caspase-1 (3866), cleaved caspase-3(9661) antibodies were obtained from Cell Signaling Technology, Inc. (Danv...
The antibodies to detect GSDME (ab215191, 1:1000) was purchased from Abcam. Antibodies targeting caspase-3 (9662S, 1:1000), cleaved-caspase-3 (9664T, 1:500), PARP (9532T, 1:1000), and MACC1 (86290S, 1:1000) were purchased from Cell Signaling Technologies. Anti-GAPDH (RM2002, 1:...
(Abcam, ab231778), anti-GSDME (Abcam, ab215191), anti-GSDMD (Abcam, ab209845), anti-RIP3(Santa Cruz, sc-374639), anti-MLKL (Abcam, ab172868), anti-STAT3 (Abcam, ab68153), anti-caspase 3 (Cell Signaling Technology, 14220), anti-caspase 8 (Cell Signaling Technology, 4790), anti-...
GSDME‑N Abcam ab215191aspase‑3 CST 14220Saspase‑8 CST 9746Sleaved caspase‑3 CST 9664Sleaved caspase‑8 CST 9748SNF‑α CST 3707SAX CST 2274SARP CST 9542S二抗/鼠二抗HPR 华安生物 HA1009 [0062] 表6实验药配制 [0063]
(#9532, 1:3000, Cell Signaling Technology), anti-GSDME (#ab215191, 1:2000, Abcam), anti-GSDMD (#ab209845, 1:2000, Abcam), anti-p62 (#ab207305, 1:3000, Abcam), anti-LC3B (#ab192890, 1:3000, Abcam), anti-caspase 3 (#ab32351, 1:3000, Abcam), and anti-GSDMC (#ap10771c,...
After that, the slides were incubated with a rabbit anti-GSDME antibody (1:100, Cat# ab215191, Abcam, MA, USA) overnight at 4 °C. Then, the donkey anti-rabbit/mouse antibody was incubated for 30 min and washed with PBS. After staining with 3,3′-diaminobenzidine (DAB), the ...
Cold atmospheric plasma (CAP) has been proposed as a novel promising anti-cancer treatment modality. Apoptosis and necrosis have been revealed in CAP-induced cell death, but whether CAP induces pyroptosis, another kind of programmed cell death is still u
The following antibodies were used for hybridization: anti-GSDME (Abcam, Cat# ab215191, Cambridge, UK, 1:1000), anti-cleaved caspase-3 (Beyotime, Cat# AC033, 1:1000), anti-cleaved PARP (Beyotime, Cat# AF1567, 1:2000), and anti-phospho-STAT6 (Beyotime, Cat# AF5950, 1:1000). β-...