Deverman团队和哈佛大学顾成华团队共同开发的AAV-BI30-Cre腺相关病毒6,将它们分别引入Gsdmdflox/flox小鼠中。通过这两种方法,他们成功实现了在脑内皮细胞中特异性敲除Gsdmd的目标。经过这样的基因操作,两种小鼠模型均失去了对LPS诱导的血脑屏障...
相关品系 Gsdmd-KO 品系名:C57BL/6Smoc-Gsdmdem1Smoc 品系状态:活体 | 目录号:NM-KO-191212 Gsdmd-EGFP 品系名:C57BL/6Smoc-Gsdmdem1(eGFP)Smoc 品系状态:精子冻存 | 目录号:NM-KI-232075 Gsdmd-Flox(2) 品系名:C57BL/6Smoc-Gsdmdtm(flox)Smoc 品系状态:精子冻存 | 目录号:NM-CKO-210102...
Pdx1Cre和Lyz2Cre小鼠来自杰克逊实验室。通过将Pdx1Cre小鼠分别与Nlrp3flox/flox和Gsdmdflox/flox小鼠杂交获得胰腺腺泡细胞Nlrp3和Gsdmd特异性敲除小鼠(Pdx1Cre Nlrp3△/△和Pdx1Cre Gsdmd△/△)。通过将lyz2Cre小鼠与Gsdmdflox/flox...
此外,作者还利用特异性靶向脑内皮细胞的AAV-BI30腺相关病毒(由美国博德研究所Benjamin E. Deverman团队和哈佛大学顾成华团队共同开发),在Gsdmdflox/flox小鼠的脑内皮细胞中表达Cre重组酶,同样实现了高效敲除脑内皮细胞的Gsdmd基因(简称AAV-GD-CKO小鼠)。 在预先给予poly(I:C)处理的条件下,GD-CKO和AAV-GD-CKO小...
通过构建 Ephx2-flox 与 Lyz2-cre 小鼠杂交,生成了 Ephx2f/f-Lyz2-cre(Ephx2CKO)小鼠模型,这种模型允许在髓系细胞中特异性地敲除 Ephx2 基因,从而积累 11,12-EET。通过 Ephx2CKO 小鼠模型,研究人员研究了 11,12-EET 的积累对肌肉损伤后修复过程的影响,包括对肌肉干细胞激活和增殖的影响。
constructed the Rosa26LSL-mGsdmd-N/+ and the Gsdmdflox/flox mice, respectively. C.W. and X.G. analysed the imaging data with supervision from Q.G. S.Z. and F.Y. provided technical assistance. L.D.J.S. and F.I.S. generated the nanobodies. B.Z. provided the bEC-cre mice. M...
Furthermore, the protein expression of Zo-1, Occludin, and Claudin-1 in two conditional knockout mice is significantly higher than that in the Gsdmdflox/flox group. Flow cytometry results show that the proportion of M1 macrophages in the mucosal lamina propria is significantly lower in two ...
pyroptosis may also occur in non-immune cells. Thus, in order to study the effect of pyroptosis in hepatocytes and innate immune cells, we generated Gsdmd-flox mice (Gsdmdf/fl) and crossed them with AlbCre+or LysmCre+mice to establish knockout mice with specific GSDMD depletion in hepatocyte...
The blood–brain barrier (BBB) protects the central nervous system from infections or harmful substances1; its impairment can lead to or exacerbate various diseases of the central nervous system2–4. However, the mechanisms of BBB disruption during infec
利用ES细胞打靶方式,在Gsdmd基因exon 3两侧分别插入loxP位点,该flox小鼠可与组织特异性Cre工具鼠交配,获得在特定细胞类型或组织中敲除Gsdmd基因的小鼠模型。与此相似的品系还有Gsdmd-Flox(NM-CKO-190060)。 *使用本品系发表的文献需注明: Gsdmd-Flox(2) mice (Cat. NO. NM-CKO-210102) were purchased from Shan...