Deverman团队和哈佛大学顾成华团队共同开发的AAV-BI30-Cre腺相关病毒6,将它们分别引入Gsdmdflox/flox小鼠中。通过这两种方法,他们成功实现了在脑内皮细胞中特异性敲除Gsdmd的目标。经过这样的基因操作,两种小鼠模型均失去了对LPS诱导的血脑屏障...
然而,该研究缺少的一个关键实验是使用中性粒细胞特异性条件敲除小鼠来明确确认中性粒细胞GSDMD在脓毒症中的因果作用。 在这项研究中,研究人员生成了嗜中性粒细胞特异性条件Gsdmd KO小鼠(Gsdmdflox/floxMRP8-Cre+)。MRP8creTg是一种...
实验结果显示,只有回补了野生型的GSDMD蛋白,而非切割位点突变型(D276A)的GSDMD蛋白,才能使Gsdmd-/-小鼠恢复对LPS刺激下血脑屏障破坏的敏感性。 此外,作者还培育了bEC-Cre;Ai14;Gsdmd+/+小鼠和bEC-Cre;Ai14;Gsdmdflox/flox小鼠,这些小鼠的脑内皮细胞特异性表达tdTomato荧光蛋白,使得他们首次能够在体内条件下观察...
中性粒细胞GSDMD缺失小鼠在LPS处理后肺组织损伤程度在体实验中,采用GSDMD中性粒细胞特异性条件敲除(GSDMD~(flox/flox) Ela-cre)小鼠及同窝对照小鼠构建急性肺损伤(acute lung injury,ALI)模型.随机分为4组:GSDMD~(WT/WT)对照组,GSDMD~(flox/flox)对照组,GSDMD~(WT/WT) LPS组以及GSDMD~(flox/flox)LPS组.检测...
pyroptosis may also occur in non-immune cells. Thus, in order to study the effect of pyroptosis in hepatocytes and innate immune cells, we generated Gsdmd-flox mice (Gsdmdf/fl) and crossed them with AlbCre+or LysmCre+mice to establish knockout mice with specific GSDMD depletion in hepatocyte...
3K-N). TUNEL assays showed that the rate of apoptotic cells in the hippocampus was greatly increased in Elacre-GSDMDWT mice under septic attack, while the situation was improved in Elacre-GSDMDflox CLP mice (Fig. 3K-L). The fluorescence intensity of IBA-1 was significantly stronger in the...
通过将lyz2Cre小鼠与Gsdmdflox/flox小鼠杂交获得髓样细胞GSDMD特异性敲除小鼠(Lyz2Cre Gsdmdf/f)。所有小鼠均为C57BL/6背景。所有小鼠饲养在无特定病原体(SPF)的条件,每笼3-5只小鼠,温度为24℃,黑暗/光照周期为12小时。 3. 随机...