实验细胞:使用LPS诱导的BV2细胞进行实验。 GSDMD抗体:选择Abcam的ab209845。 NLRP3抗体:选择Abcam的ab263899。 跑胶操作:使用12%的BBI预制胶或雅酶12%的PAGE胶进行电泳。建议使用预制胶,效果更佳。电泳时先以60V电压运行,然后转至100V继续运行。 转膜步骤:每张膜以220mA的电流进行转膜,持续120分钟。 封闭操作:使用...
abcam代理商之产品ab209845-重组Anti-GSDMD抗体[EPR19828] (ab209845)产品的应用范围: 【蛋白质/抗原/多肽】球蛋白|封闭肽|人蛋白和抗原|小鼠蛋白和抗原|蛋白和抗原|病毒蛋白和抗原|植物蛋白和抗原|其它蛋白和抗原|细胞质蛋白|总蛋白|膜蛋白|核蛋白|细胞裂解和提取物|线粒体蛋白|细胞裂解|组织提取|重组细胞因子 ...
品牌:Abcam 所属分类:一抗 货品编号: Abcam.ab209845 品牌:Abcam 品名:重组Anti-GSDMD抗体[EPR19828] 规格:10ul/支 产品描述: Product name Anti-GSDMD antibody [EPR19828] See all GSDMD primary antibodies Description Rabbit monoclonal [EPR19828] to GSDMD ...
Western blotting analysis was performed as we previously described51. Primary antibodies were used against mouse caspase-1 (#ab179515), Gsdmd, Gsdmd-N (#ab209845) (Abcam Cambridge, MA, USA), NLRP3 (#15101), ASC (#67824) (Cell Signaling, Boston, MA, USA); for these specific proteins, ...
Candida albicans is the most common cause of fungal sepsis. Inhibition of inflammasome activity confers resistance to polymicrobial and LPS-induced sepsis; however, inflammasome signaling appears to protect against C. albicans infection, so inflammasome
Candida albicans is the most common cause of fungal sepsis. Inhibition of inflammasome activity confers resistance to polymicrobial and LPS-induced sepsis; however, inflammasome signaling appears to protect against C. albicans infection, so inflammasome