Preparation of Rat Brain Cortex Mitochondria—Mitochondria prepared from rat brain cortex (2-month-old CD rats; Charles River Laboratories) were purified on sucrose gradient as reported previously (43) and then resuspended in an isotonic medium containing 2 mm HEPES, pH 7.4, and 0.32 m sucrose ...
(1988). The three known endothelins ET-1, -2 and -3 are structurally similar to sarafotoxins from snake venoms. ET-1 is the major isoform generated in blood vessels and appears to be the isoform of most importance in the cardiovascular system with a major role in the maintenance of ...