gRNA targeting sequencegRNA靶向序列，即PAM序列之前的20个碱基，扮演着crRNA的作用。该序列被克隆到gRNA...
gRNA scaffold sequence 是gRNA内负责Cas结合的序列,不包括用于引导Cas9靶向DNA的20bp间隔/靶向序列。 4.CaMV enhancer(CaMV 增强子) 增强子是DNA上一段能与蛋白质结合的区域。与蛋白质结合后,基因的转录作用会增强。 5.Kozak sequence Kozak 序列通常为ACCACCAUGG,位于起始密码子AUG的两侧,通过与翻译起始因子的结合...
By introducing an A/G mixed capture sequence into the gRNA scaffold, we demonstrate that gRNA transcripts could be directly reverse transcribed by poly (dT) primer together with the endogenous mRNA, followed by high-content molecular phenotyping in scRNA-seq (Direct-seq). With this method, the ...
ON-OFF-Insert-Scaffold: 2 AarI restriction sites, 1 conventional gRNA scaffold sequence, and 7 consecutive thymines (T) were synthesized and cloned into pET-28a(+), resulting in the ON-OFF-Insert-Scaffold vector (kanamycin resistance marker). lenti-Cas9-Blast: lenti-Cas9-Blast (Addgene #529...
CRISPR-Cas9 is a powerful DNA editing tool. A gRNA directs Cas9 to cleave any DNA sequence with a PAM. However, some gRNA sequences mediate cleavage at higher efficiencies than others. To understand this, numerous studies have screened large gRNA librari
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gRNA_AAVS1-T2质粒价格1200元,gRNA_AAVS1-T2(爱迪基因 41818 )质粒图谱(map),质粒序列(Sequence)见下文,质粒抗性为卡那霉素,测序引物为T7。gRNA_AAVS1-T2质粒是哺乳细胞表达,CRISPR。
This yields a modified vector, pgRNA1 containing the U6 promoter, followed by the 5′G of the gRNA sequence and the scaffold sequence, but lacking the targeting sequence. For lentiviral vector construction, the vector pLKO.1 (Addgene plasmid 10878) was modified to insert the gRNA promoter and...
gene, species, sequence, Addgene plasmid ID (if we have one associated with the gRNA), application, cas9 species, PubMed ID, and depositor. TheCRISPR applicationswe currently have data for are: cut, activate, interfere, visualize, nick, purify, tag, scaffold, CRISPR-display, and dCas9-...
pUC119-gRNA质粒价格1200元,pUC119-gRNA(爱迪基因 52255 )质粒图谱(map),质粒序列(Sequence)见下文,质粒抗性为氨苄青霉素,质粒大小为3162 bp,测序引物为见下文。pUC119-gRNA质粒是CRISPR;植物表达。