已有报道表明GRF-GIF嵌合体蛋白可显著提高小麦、西瓜等再生能力,但其在大豆再生中的研究报道较少。 JIPB近日在线发表了东北农业大学陈庆山课题组题为“A highly efficient soybean transformation system using GRF3-GIF1 chimeric protein”的研究论文 (https://doi.org/10.1111/jipb13767),介绍了利用GRF3-GIF1嵌...
分子植物育种 Molecular Plant Breeding ISSN 1672-416X,CN 46-1068/S 《分子植物育种》网络首发论文 题目: GRF-GIF 嵌合体在草莓遗传转化中的应用 作者: 郭苏平,田美萍,丁静 网络首发日期: 2022-05-11 引用格式: 郭苏平,田美萍,丁静.GRF-GIF 嵌合体在草莓遗传转化中的应用[J/OL].分子植物育种. https:/...
GRF-GIF chimeras could be a game-changer in plant transformation and gene editing.doi:10.1016/j.tplants.2020.12.001Guangbin LuoMichael PalmgrenTrends in Plant Science
利用农杆菌介导法将两个GRF-GIF嵌合基因转化森林草莓叶片外植体.观察愈伤组织形成和分化状况,并计算芽再生率和遗传转化效率.结果发现,2对GRF-GIF嵌合基因均可提高森林草莓愈伤组织的芽再生率.其中GRF5-GIF1的促进效果更明显,其芽再生率和遗传转化效率为对照的2.25倍.总之,本研究为提高草莓遗传转化效率提供了一种新...
最后,我们显示了双子叶植物GRF-GIF嵌合体可提高柑橘的再生效率,表明该策略可应用于双子叶植物。 The potential of genome editing to improve the agronomic performance of crops is often limited by low plant regeneration efficiencies and few transformable genotypes. Here, we show that expression of a fusion...
GRF1homologs. None of the authors of this manuscript is part of the KWS/BASF patent or is related to these companies. The KWS/BASF patent focuses on a different cluster ofGRFgenes than the one described in our study and does not incorporate theGIF1cofactor or the generation ofGRF–GIF...
当使用西瓜内源ClGRF-GIF嵌合基因转化TC时,转化效率提高到了47.02%。研究人员继续突变了ClGRF4基因内部的miR396的作用位点得到ClrGRF-GIF,该嵌合基因可进一步将遗传转化效率提高到67.27%。鉴于ClrGRF-GIF在西瓜遗传转化中的显著效果,研究...
另外,研究人员还突变了GmGRF3基因的miR396b作用位点,得到了rGRF3-GIF1,更是实现了约4.67倍的转化效率提升。这些成果不仅阐明了GRF-GIF蛋白在大豆再生中的重要作用,同时为大豆基因编辑提供了强有力的支持,特别是在CRISPR/Cas9技术的应用上,达到了前所未有的高效性。
GRF proteins [7]. The C-terminal region has transactivation activity and is rich in Gln (Q) and Gly (G), and thus, the C-terminal of GIF proteins is called the QG domain [6]. GIF proteins also have important biological functions. The overexpression ofGIFgenes can promote organ growth ...
Interestingly, we found that, in addition to the leaf size, the GRF system also affects the organ longevity. Genetic and molecular analysis revealed that the functions of GRFs in leaf growth and senescence can be uncoupled, demonstrating that the miR396-GRF-GIF network impinges on different ...