A C18 reversed–phase packing column (Prodigy ODS (3), 4.6 × 150 mm, 5 μm; Phenomenex, Torrance, CA, USA) was used for the separation and the column was thermostated at 30 ± 1°C using a Jetstream2 Plus column oven. The UV/Vis acquisition wavelength was set in the range of ...
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(Phenomenex, Inc., Torrance, CA, USA). The temperature of the analysis was adjusted to 40 °C and the eluents used were (A) 0.5% aqueous formic acid and (B) 0.5% formic acid in acetonitrile/water (6:4). The injection volume was 20 μL and the flow rate was 1 mL min−1. ...
(Phenomenex, Inc., Torrance, CA, USA). Quantification was done with calibration curves (0–50 μg mL−1) constructed with rutin (R2= 0.9990), luteolin-7-O-glucoside (R2= 0.9980), apigenin (R2= 0.9999) and oleuropein (R2= 0.9990). Luteolin-7-O-glucoside and apigenin were quantified ...
(250 × 4.6 mm i.d., particle size 5 μm, Agilent Technologies, Palo Alto, CA, USA) protected by a C18Guard Cartridge (4.0 × 2.0 mm i.d., Phenomenex, Torrance, CA, USA). HPLC separation was accomplished by using a binary mobile phase composed of (solvent A) H2O/formic acid 10...
The extracts of fruits and vegetables indicated inSection 2.2were analyzed by HPLC with diode array detection (DAD). A Kinetex C18 column (100 mm × 4.6 mm of internal diameter and 2.6 µm particle size) from Phenomenex (Torrance, CA, USA) was used. The mobile phase was composed of 0.1...
This work describes an untargeted analytical approach for the screening, identification, and characterization of the trans-epithelial transport of green tea (Camellia sinensis) catechin extracts with in vitro inhibitory effect against the SARS-CoV-2 papa
(Phenomenex, Torrance, CA, USA). Gradient elution was applied using acetonitrile (A) and water acidified with 1% formic acid in d-water (B), at flow rates of 1 mL/min, at the following order: 10% to 25% A (0–10 min); 25% to 60% A (10–20 min); 60% to 70% A (20–...