Reports that epidermal growth factor (EGF) stimulation of canine epithelial cells induced a rapid and transient association of the protein GRB2 with dynamin, a guanosine triphosphatase that regulates endocytosis. Internalizing of EGF receptors into the endocytotic compartment; Degrading of receptors in ...
Grb2 regulates internalization of EGF receptors through clathrin-coated pits. Mol Biol Cell. 2003;14(3):858–70. Article CAS PubMed PubMed Central Google Scholar Yamazaki T, Zaal K, Hailey D, Presley J, Lippincott-Schwartz J, Samelson LE. Role of Grb2 in EGF-stimulated EGFR ...
PLCɣ isoforms are not required for activation of Erk in DG75 B cells. (A) Ca2+mobilization profiles of parental DG75 cells (wt, black curve) and sublines lacking either PLCɣ1 (turquoise curve) or PLCɣ2 (light blue curve) or both (dark blue curve) upon stimulation of the endog...
Human Epidermal Growth Factor 2 (HER2) is an oncogenic receptor tyrosine kinase (RTK) belonging to the Epidermal Growth Factor (EGF) receptor family1. HER2 is overexpressed in 15–20% of breast cancer (BCa) patients (HER2+), and this is associated with poor prognosis2. HER2 overexpression in...
These 185-kDa transmembrane glycoproteins are activated by EGF, EGF-like, or neuregulin ligands to form homodimers and heterodimers, and the intracellular tyrosine kinase domain then activates several important oncogenes downstream in the signaling cascade. ERBB2 is unique in lacking a ligand; its ...
Upon stimulation of appropriate cells with any of a number of receptor tyrosine kinase ligands, including epidermal growth factor (EGF), hepatocyte growth factor (HGF), platelet-derived growth factor (PDGF), nerve growth factor (NGF), and insulin or insulin-like growth factor 1 (IGF-1), Gab...
In many cell systems, the SH3 domains of Grb2 bind to Sos whereas the interaction between the Grb2 SH2 domain and tyrosine-phosphorylated molecules such as the epidermal growth factor (EGF) receptor or Shc may regulate the function and cell localization of Sos (27, 28, 29, 30). Studies...
EGFR was one of the most important RTKs in recruiting Grb2-Sos1 complex to regulate ERK activity in response to extracellular EGF stimulation [8, 22]. Sos1, as described before, was the famous guanine nucleotide-exchange factors in directing exchange of Ras-GDP to Ras-GTP to activate ERK ...
Three GAREM2 tyrosines (Tyr-102, Tyr-429, and Tyr-551) are phosphorylated upon EGF stimulation and are necessary for binding to Grb2. Furthermore, GAREM2 and Shp2 regulate Erk activity in EGF-stimulated cells. These characteristics are similar to those of GAREM1. GAREM2 is expressed in ...
We found that through the interaction between NoxO1 and growth receptor–bound protein 2 (Grb2), the Casitas B–lineage lymphoma (Cbl) E3 ligase was recruited, leading to decreased NoxO1 stability and a subsequent reduction in ROS generation upon epidermal ...