但是,关于GRB2和SOS1这样的分子是如何发生聚集和离散,引起宏观液滴的形成和消失的,在分子原子水平上几乎没有弄清楚。 研究没有进展的原因之一还在于,由于GRB2和SOS1具有比普通蛋白质更柔软的区域,因此结构生物学研究中采用最多的x射线晶体结构分析和低温电子显微镜等解析法难以适用。 随着对GRB2和SOS1的蛋白质间相...
Grb2是生长因子受体结合蛋白2,又叫Ash蛋白。该蛋白参与细胞内各种受体激活后的下游调节。它能够直接与激活的表皮生长因子受体磷酸化的酪氨酸结合,参与EGF受体介导的信号转导,也能通过与Shc磷酸化的酪氨酸结合间接参与由胰岛素受体介导的信号转导。Grb2能够同时与Shc、Sos结合形成Shc-Grb2-Sos复合物,并将Sos激活,激活的...
Association of the DF3/MUC1 breast cancer antigen with Grb2 and the Sos/Ras exchange protein. Cancer Res 1995;55:4000-3.Pandey P, Kharbanda S, Kufe D. Association of the DF3/MUC1 breast cancer antigen with Grb2 and the Sos/Ras exchange protein. Cancer research 1995;55:4000- 4003...
先给我们讲的是LAT-Grb2-Sos1这一段信号,作者在体外构建了这一段信号体系:讲磷酸化的LAT(pLAT)装在磷脂双分子层上,而后加入Grb2和Sos1(注意pLAT、Grb2、Sos1之间的互作位点)镜下观察,发现蛋白聚集成簇,加入磷酸酶PTP1B后蛋白簇逐渐消失 点击添加图片描述(最多60个字)编辑 未磷酸化的LAT加入Grb2、Sos1后形...
It contains two SH3 domains and an SH2 domain. GRB2 plays a crucial role in connecting the signaling pathways of T cells and B cells to the Ras/MAPK pathway, by recruiting the guanine nucleotide exchange factor Sos. Additionally, GRB2 can also interact with other adaptor proteins associated...
( 1996 ). Gastrin induces tyrosine phosphorylation of Shc proteins and their association with the Grb2/Sos complex . FEBS Lett. , 378 , 74 – 78 .Seva C, Kowalski-Chauvel A, Blanchet JS, Vaysse N, Pradayrol L. Gastrin induces tyrosine phosphorylation of Shc proteins and their association...
Grb2 is an adaptor protein that recruits Ras-specific guanine nucleotide exchange factor (GEF), such as Son of sevenless 1 (SOS1), to the plasma membrane. SOS1 exchanges GDP by GTP, activating Ras. Grb2 consists of an SH2 domain in the middle and flanks nSH3/cSH3 domains at both ...
The SH2 domain binds to phosphotyrosine residues in RTKs such as PDGF and EGF, non-RTKs such as Bcr/Abl and FAK, and docking proteins such as FRS-2 and Gab1. The SH3 domains associate with proline rich motifs on the guanine nucleotide releasing factor, Sos, stimulating GTP binding to...
GRB 2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2-Sos complex then binds the EGF receptor. These interactions play a critical role in the ...
Recommended positive controls include HeLa cells and tonsil tissue. 靶标信息 GRB 2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2-Sos complex ...