The present invention describes a novel mechanism of adhesion by flagellated Gram-negative bacteria such as enterotoxigenic Escherichia coli (ETEC), where the bacteria secretes a protein, EtpA which binds to the conserved region of the flagellin protein located at the tip of the flagella. The prese...
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aeruginosa R22 (standardised to 107 CFU/mL) in a Greiner glass-bottomed optical 96-well plate. The plate was then wrapped in parafilm and incubated (37 ◦C, 20 rpm) for 24 h. The supernatant was carefully removed and replaced with 10% v/v LIVE/DEAD stain in PBS prior to imaging. ...