The term “Gram stain test” as used herein refers to a staining procedure which differentiates bacteria into two groups (Gram positive and Gram negative) on the basis of differences in their cell-wall structure. In brief, bacteria are heat-fixed to slides, stained with a purple dye (crystal...
coli which exhibited a reduced sensitivity to deoxycholate, an inability to grow at temperatures above 32.5° C., and a decrease in LPS staining intensity (Schnaitman et al., 1993, supra; Karow et al., 1992, J. Bacteriol. 174:7407-7418). Karow et al. further noted that at between ...