5. METHODFix dry smear as explained above in the section “Drying and Fixing smears”. (1) Cover the fixed smear with crystal violet stain for 30 - 60 seconds. (2) Wash off the stain with clean water. (3) Tip off all the water, and cover the smear with iodine for 30 - 60 secon...
Assorted References antibiotics In antibiotic: Categories of antibiotics gram stain test In Gram stain In bacteria: The Gram stain plant diseases In plant disease: General characteristicsArticle Contributors Primary Contributors Kara Rogers Kara Rogers is the senior editor of biomedical sciences at Encyclo...
Noun1.Gram stain- a staining technique used to classify bacteria; bacteria are stained with gentian violet and then treated with Gram's solution; after being decolorized with alcohol and treated with safranine and washed in water, those that retain the gentian violet are Gram-positive and those...
generally appear dry, and Gram stain shows large, highly vacuolated, pleomorphic bacilli; growth on MacConkey agar at temperatures higher than 40°C usually is negative. In contrast, colonies of Roseomonas spp. are mucoid in culture and show nonvacuolated, coccoid bacilli on Gram...
Aerobic gram-positive bacilli are a group of bacteria identified by metabolic processes and stain color. Explore the defining characteristics of aerobic gram-positive bacilli, types of these bacteria, and tests used to identify them in a lab. ...
1 µM Maleimide 560 LIVE (Abberior) was added and incubated 30 min at 37 °C with low agitation (300 rpm) to stain the DNA. Cells were washed again twice and put on agarose pad 1:5 LB-PBS (1% agarose). Cells were then imaged using the Nikon Ti-2 Eclipse inverted ...
Gels deteriorate in a relatively short time, however, and the dyes used to stain nucleic acids in gels deteriorate even more rapidly. This mandates archiving an image of the gel electronically and/or producing a tangible photographic record (an electrophoretogram). This also applies to the ...
PI. PI is a red fluorescent nucleic acid stain that intercalates with DNA and when bound, fluorescence is enhanced up to 30-fold upon penetration of cells with disrupted membranes and is excluded from viable cells. NT (No treatment), Cipro (Ciprofloxacin), H (Hoechst dye control), PB (...
Bacterial membrane integrity was further assessed using SYTO 9/PI Live/Dead Bacterial Double Stain Kit (Pimchan, Tian, Thumanu, Rodtong, & Yongsawatdigul, 2023). The logarithmic phase bacteria were harvested, washed thrice with saline, and diluted to 1−5 × 105 cfu/mL. The bacterial sus...
Silver phosphate and its composites have been attracting extensive interest as photocatalysts potentially effective against pathogenic microorganisms. The purpose of the present study was to investigate the mechanism of bactericidal action on cells of op