The method comprises the following steps: evenly coating a slide with a to-be-detected material at room temperature; drying and fixing; dropwise adding a primary dye on a smear; dyeing and pouring out the primary dye and then directly dropwise adding a counter-stain; dyeing and washing one ...
The sensitivity of the Gram stain procedure is low. Sometimes, you may fail to see the organism in Gram Stain smear, but the same clinical specimen may yield organisms when cultured. To be visible on a slide, organisms that stain by the Gram method must be present in about 104to 105organ...
Gram staining is a method used to distinguish between types of bacteria. It determines if bacteria are present or not and identifies phenotypic characteristics of bacterial samples. Which of the steps in the Gram stain is the most critical and why? The most important step of gram staining is ...
A simplified Gram's staining method is described. The decolorization step of 95% alcohol in the original Gram's stain was omitted, and sub-stituted by a counter stain prepared with 0.4% fuchsin in alcohol which acts as both decolorization and counter staining in the simplified Gram staining me...
Gram Stain | Purpose, Theory & Procedure from Chapter 4 / Lesson 3 75K Learn how to perform a Gram stain, and see the purpose of Gram staining. Know Gram stain steps, and a description of what happens at each step in the Gram stain. Related...
Explain the steps of the staining method for acid-fast stain. In four steps, describe the simple staining procedure. What would happen if a student omitted the alcohol wash step when doing the Gram stain procedure? What are some potential problems with the Gram Stain?
The Gram staining method, and the differentiation between positive and/or negative status, depends on the existence of a permeability barrier in bacteria based on the chemical and physical properties of the cell wall. The reaction to Gram staining could be negative, strongly positive or weakly ...
Gram's stain: the key to microbiology. Gram's stain remains one of the most valuable methods we have for identifying isolates accurately and rapidly. Despite our long-standing familiarity with this method, it still warrants careful attention every step of the way--from prepar... R Mcclelland...
Crystal VioletPrimary stain IodineMordant or dye fixative Acetone and/or alcohol (95%)Decolorizing agents SafraninCounterstain The step-wise procedure involved in the Gram staining method is listed below: Prepare the smear of bacterial suspension on a clean glass slide. ...
By making comparison between three-step method and four-step method of Gram stain,the author found that the stain progress of three-step method is simple,and is easy to hold decolor time. 对革兰氏染色三步法和四步法进行了试验比较,发现三步法染色过程操作简单,脱色时间易于掌握,便于学生操作和控制...