of a score ≥7 in the Gram stain =-=[25]-=-, and by the Amsel’s method, that is the presence of three or more of the following criteria [26]: (i) “clue-cells” in the Gram stain, (ii) pH ≥ 4.5, (iii) positive fishy-odor test, and (iv) thin and ...Nugent RP, ...
Methods: Women attending genitourinary medicine (GUM) clinics, as part of a multicentre study, were diagnosed as having BV if three or more of the following criteria were present; homogeneous discharge, elevated vaginal pH, production of amines, and presence of "clue" cells. Women with less ...
Flood air-dried, heat-fixed smear of cells for 1 minute with crystal violet staining reagent.Please note that the quality of the smear (too heavy or too light cell concentration) will affect the Gram Stain results. Wash slide in a gentle and indirect stream of tap water for 2 seconds. Fl...
E. coli MG1655 cells were spotted onto a 1.5% agarose pad containing dynobactin A (8x MIC), the membrane stain FM4-64 10 μg mL-1 (false-colored in magenta), and membrane permeabilization stain Sytox Green 0.5 μM (false-colored in green). Cells were incubated at 37 °C...
Positive criteria for a Gram stain included greater than 10 white blood cells per high-power field, gram-negative intracellular/extracellular diplococci (suggesting N gonorrhoeae), clue cells (suggesting T vaginalis), or direct visualization of T vaginalis organisms. DNA probes were used as the ...
Clinical data consisting of vaginal pH, "whiff test," clue cells, and appearance of the vaginal discharge (Amsel criteria) were compared with the vaginal fluid Gram stain (Nugent criteria) for the diagnosis of bacterial vaginosis. Results: The sensitivity and specificity of the Gram stain ...
(Whiff test) and presence of clue cells Amsel criteria were compared with Gram stain diagnosis One hundred two patients were diagnosed with bacterial vaginosis When compared Gram stain diagnosis of bacterial vaginosis as the standard method, Amsel criteria had sensitivity of 78% a specificity of 88...
Endocervical smears were also collected for Gram stain. The number of polymorphonuclear leucocytes (PMN) per high power field (HPF), presence of bacteria, yeast, red blood cells, and clue cells were recorded. Clinical signs of cervicitis were also documented. Results:N gonorrhoeae was isolated ...
Endocervical smears were also collected for Gram stain. The number of polymorphonuclear leucocytes (PMN) per high power field (HPF), presence of bacteria, yeast, red blood cells, and clue cells were recorded. Clinical signs of cervicitis were also documented. Results: N gonorrhoeae was isolated ...
Clinical data consisting of Whiff test, clue cells, and appearance of vaginal discharge (Amsel criteria) were compared with vaginal fluid Gram stain (Nugent criteria) for diagnosis of bacterial vaginosis.Results: The prevalence of bacterial vaginosis was 25.1%. The sensitivity and specificit...