WT-96 96wells Color Touch Screen Gradient PCR thermal cycler Applications: This gradient Thermal Cycler is ideal for thermal cycling and protocol optimization in molecular biology, medical, food, genetic testing and etc Features: 1. Excellent amplication due to high quality Semiconductor ...
PCRcontinuous-flowthermal gradientDNAamplificationmicrofluidicsXurographyrapid prototypingA new continuous-flow PCR microchip has been developed that operates by cycling a prepared sample within a spatial temperature gradient. This design allows for minimal thermal residence times - a key feature of the ...
PCR products were analysed on a CEQ2000 sequencer (Beckman Coulter; denaturation at 90 °C for 2 min, injection at 2.0 kV for 30 s, separation at 6.0 kV for 45 min) along with the manufacturer's internal size standard. We screened the resulting fragment length data using ...
In total, 12 genes were selected for validation of their expression profiles by real time reverse transcription-quantitative PCR. Two of these genes displayed either a multi-banding pattern on agarose gel electrophoresis or unsuccessful PCR amplification and were removed from the analysis. For the oth...
(< 30 bp –base pairs–) was accomplished by keeping the base with the higher quality score. The CUTADAPT software 1.395was used to remove sequences that did not contain the PCR primers (allowing up to 2 mismatches) and sequences that ended up between 293 and 333 bp for the COI ...
To reduce the effect of PCR drift, amplicon PCR was run in duplicate. The V4 hypervariable region of the 16S rRNA gene was amplified using the well-described F515/R806 primer set (Caporaso et al., 2011). The reaction mix contained 1× Platinum High Fidelity buffer (Thermo Fisher ...
Graphical abstractSchematic presentation of an open system with a thermal gradient designed for PCR optimization. The exact temperature-time course of the sample is monitored in real-time throughout the thermal cycling. The method ensures significant time-savings and a reduction in reagent use during...
Reactions were run in thermocyclers manufactured by either a PTC-100 programmable thermal controller (MJ Research, Waltham, MA, USA) or a PCR Express (Thermo Hybaid; Thermo Fisher Scientific, Pittsburgh, PA, USA) thermalcycler. For primer sets targeting Bacteria (8F with 1492R) and Eukaryotes...
PCRthermal cyclingEasy-to-use thermal cycling for performing rapid and small-volume DNA amplification on a single chip has attracted great interest in the area of rapid field detection of biological agents. For this purpose, as a more practical alternative to conventional continuous flow thermal ...
Overall, the impact of design and operating parameters are observed to be least on thermocycling profile at low Reynolds number (≤0.37 Re). However, in addition to the primary metric advantages of CF-PCR, one heater chip design helps in minimizing the thermal crosstalk effects by a factor ...