separationsoftware packages/ PREOPT-W simulation programoff-line optimizationbinary gradient separationsHPLCOff-line gradient optimization by means of the PREOPT-W simulation program is described and illustrated
Linear gradients have been used successfully in many applications [14], including one-step isolation of a novel bio-active compound from an untreated plant extract [12]. When CCC is used to purify a compound, it is difficult to extrapolate from the gradient separation to the isocratic ...
usually increasing flow to get the later eluters to come out more quickly. The column usually chosen for additive analysis is either an octadecylsilane (C18) or Octylsilane (C8) column, ~15 cm in length. An example of a reverse phase gradient (with flow program) separation of a series of...
The pIsep separation utilizes an external pH gradient and the charged variants are resolved based on pI differences. In this study, an acidic variant co-eluted with main peak on traditional salt-gradient cation-exchange chromatography (CEX) is separated by pIsep and the fraction was collected ...
Recoveries of 95 and 97% were obtained for metabolite 17 and 1 using a diethyl ether extraction scheme in both blood and bile. Separation of cyclosporine and its metabolites in whole blood and plasma indicate that there is a significant temperature-dependent partitioning of these compounds between ...
In this work, a simple, rapid, and reliable HPLC-DAD method was described for the simultaneous determination of the three antihypertensives. Chromatographic separation was achieved using Zorbax SB-C8 column with gradient elution of the mobile phase composed of 0.025 M phosphoric acid and acetonitrile...
The mechanism of separation of solutes under gradient elution conditions has been studied. Separation of a mixture of soybean phospholipids (phosphatidylethanolamine, phosphatidylinositol, and phosphatidylcholine) in the ternary mobile phase: methanol, hexane, isopropanol was considered as an example. An ana...
Impact of different GDV on a gradient separation using a Vanquish Core HPLC system. The lowest GDV can be achieved by an idle volume setting of 0 µL while switching the method transfer loop out of the flow path. The highest GDV can be implemented by an idle volume setting of 230...
Techniques to Measure SCBD in Polyolefins. This is Chapter 2 of 3 in a series of videos that explain what is Short Chain Branching Distribution, techniques for measuring it, and some applications. Watch now Webinar: Modern Separation Techniques for Polyolefin Microstructure Characterization Webinar ...
Gradient elution in HPLC refers to the technique of altering the composition of the mobile phase during the course of the chromatographic run. While the majority ofHPLC separations utilize isocratic elution conditions, i.e. the mobile phase remains constant over the course of the separation, gradie...