The number of ACF per colon and the number of crypts per ACF were counted under a light microscope at 40X magnification in duplicate by two readers who were blinded to sample origins. Statistical analysis. The results are expressed as the mean ± s.e.m. Statistical significance ...
Interleukin-10 (IL10) is an anti-inflammatory cytokine that helps to regulate the immune response and induces macrophages to polarize toward anti-inflammatory phenotypes [16]. Owing to the direct inhibition of the pro-inflammatory cytokine [17], IL10 can also reduce the proliferation of astrocytes...
Subsequently, slides were fixed with 4% paraformaldehyde for 10 min and incubated with Alexa 488-labeled goat-anti-mouse and Alexa 555-labeled goat-anti-rat (Molecular probes; 1:500) for 1.5 h. DNA fibers were imaged with the Zeiss AxioObserver Z1 inverted microscope using a 63x objective ...
We used a custom-built inverted fluorescence microscope setup (Supplementary Figure 1b) to compare the performance between the sCMOS camera and the industry-grade CMOS camera (Fig. 2a) for single emitter localization. By equally dividing the fluorescence signal by a 50/50 beamsplitter cube to bo...
The duration of mitosis of tumor cells is prolonged or even damaged when observed under microscope. Under the intervention of TTFields, immunohistochemical staining showed tumor cells undergoing abnormal mitosis, which is due to intervention by TTFields in spindle formation. These abnormalities were simila...
Sieves and screen testing kit contains Densimeter, PIN Gauge, 8x Pen microscope 50x with scale magnifier, with scale, 30x magnifier, GO-No-gauge, advance 90 x microscope with special measuring program. Comes with complete set of certificates. ...
Slides were counterstained for DNA with 4′,6-diamidino-2- phenylindole (DAPI) and mounted with ProLong Gold antifade reagent (Life Technologies). Scoring of centro- some phenotypes was performed using a Nikon Eclipse Ti inverted microscope, 100x objective, and CoolSNAP HQ2 charge-coupled device...
The study represents an exposure to “real-life” particles in the relevant route of exposure in humans after ingestion of either food or pharmaceuticals. In general, the exposure to E153 and E171 is higher in children than adults and elderly [9, 10]. Nevertheless, the elderly, patients ...
This analysis was performed on 5 photomicrographs at 400× magnification for each case, using the LEICA DM500 microscope equipped with the LEICA ICC50 W camera. Following software calibration, optical density (OpDe) measurements were taken to quantify the loss of SMARCB1 and SMARCA4 expression ...
From the perspective of a pathologist visualizing stained tissue sections under a microscope, HGSOC tumours typically feature solid masses of cells (Figure 1A) with slit-like fenestrations (Figure 1B) [9]. In some areas, the tumours often have a papillary (Figure 1C), glandular (Figure 1B...