The DNase I digestion reaction was carried out by adding a total volume of 10 μL solution containing approximately 0.015 units of DNase I (Promega, USA) and 100 nmol CaCl2 and further incubating for 1 min at 37 °C, then stopped by adding a total volume of 140 μL stop ...
The number of units are standardized as per 103 producing cells. LacZ reporter gene expression A×2 cells and grlJ- cells were transformed using electroporation with the ecmO-Gal and pspA (D19)-Gal plasmids separately. The transformants were selected with 4 μg/ml G418 for wild type cells...
Each bar denotes the mean relative light units (RLU)± S.E., n = 3, p > 0.05. E–H, PBMC T cells were pretreated with IPI-549 for 1 h and then stimulated with 1 mg/ml plate-bound OKT3 and soluble CD28 5.5 h prior to addition of actinomycin D. mRNA levels of the indicated ...
Purified NK cells were cultured in medium containing 200 units/ml IL-2. Antibodies Antibodies used were from as follows: anti-human TIGIT PE (eBioscience, San Diego, CA, USA); anti-PVR; Perforin antibody (ab47225) from Abcam (Cambridge, UK); anti-phosphotyrosine 4G10 Platinum 05–1050...
The titer of Ad-GRK2-S29A obtained was 1.0 × 1011 plaque-forming units/ml, and the adenoviral particles were used at a multiplicity of infection of 10 to infect cultures of cardiac myocytes. RNA Interference for PKCα Knockdown of PKCα was accomplished using an RNA interference (RNAi) ...
This is performed by injecting a pre-determined dose of insulin (0.5 Units/kg in saline) or glucose (1 gm/kg in saline) and changes in glucose levels are monitored by tail vein bleed every 30 minutes. The compounds that lead to decreased levels of glucose after insulin injection and after...
胚胎表面上皮细胞(surface epithelium,SE)是源自于外胚层侧面的单层上皮,可进一步分化为眼表上皮、皮肤上皮及其他表面外胚层衍生组织【1】,SE发育异常将导致一系列其衍生组织的病变。谱系命运决定依赖于染色质的表观遗传结构和顺式调控网络协同配合【2】。然而,SE的谱系决定过程、调控网络和关键转录因子仍不清楚。
Table 2. Comparison of the direct cytotoxicity of NK-92 transfectants against K562 cells by using the lytic units at 30% specific lysis (LU30) for 107 effector cells. For the two most common FCGR3A allelic variant combinations in the European population, NK-92 LL_VF and NK-92 LL_FF,...
The regeneration mechanism is probably based on the degradation and regeneration of intracellular proteins, including the sensing and reporting units. The functions of the sensor cells could differ based on cell type. In order to test sensor cell development in various cell types, we introduced ...
Results from our study suggest including at least 1 fossil per 10 operational taxonomic units (OTUs) in divergence dating analyses.EBSCO_AspSystematic Biology