Applications Tested: This 4NZBRGO antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in...
Increased blood lactate is sufficient to induce the cachectic phenotype Lactate induces cancer cachexia via GPR81 Role of adipose tissue in the development of cancer cachexia Lactate induces WAT browning via GPR81–Gi–Gβγ–RhoA/ROCK1–p38 cascade Discussion Methods Data availability Change history ...
Results GPR174 knockout enhances blood flow recovery in HLI mice To determine the possible role of GPR174 in blood flow recovery, we used Laser Doppler imaging to evaluate blood flow in wild-type (WT) and GPR174-knockout (Gpr174−/Y) mice after HLI (Supplementary Fig. 1a, b). The ...
Results indicated that blood pressure in the Ang II group was significantly higher than in the control group. Notably, GPR39 knockout markedly attenuated the Ang II-induced elevations in systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean arterial pressure (MAP) (Fig. 2a–...
Unpaired two-tailed Student's t test was performed. (B and C) Plasma total GLP-1 (B) and GIP (C) levels in response to an oral glucose load (3 g/kg) in male inducible intestinal Gpr17 deficient mice (iKO, n = 6) and WT mice (n = 11). Tail blood samples were collected at ...
The phagocytic ability of macrophages toward live cancer cells was evaluated by a luminescence-based long-term phagocytosis assay as we previously described71. Specifically, luciferase-expressing Raji cells (ATCC, Cat# CCL-86) were co-cultured with BMDMs isolated from mouse blood for 24 h in th...
Blood was kept on ice. Within 1 h after sampling, blood was centrifuged at 5000 rpm for 10 min at 4 °C. Immediately after centrifugation, the resulting plasma samples were collected into polypropylene tubes and were kept frozen at −20 °C pending bioanalysis. Plasma samples were assayed ...
In GPR55-deficient and wild-type littermate control mice, in vivo blood pressure measurement and isolated resistance artery myography were used to determine GPR55 dependence of atypical cannabinoid-induced haemodynamic and vasodilator responses.Atypical cannabinoids O-160...
(FO) cells. MZ cells are positioned at the splenic red pulp border to scan the blood for circulating antigens, and FO cells interact with T follicular helper (Tfh) cells to initiate the germinal center (GC) response9. This culminates in the final stage of B cell maturation, which ...
Results Cryo-EM structures of GPR21-mGs and GPR21-mG15 In order to test whether GPR21 can signal through Gs and G15/16 as reported8,9, and to obtain stable GPR21-G protein complex for structural investigation, we first performed bioluminescence resonance energy transfer (BRET) assays to me...