gp41‐1 inteinorthogonal split inteinprotein engineeringprotein splicingProtein trans ‐splicing catalyzed by split inteins has increasingly become useful as a protein engineering tool. We solved the 1.0 ‐resolution crystal structure of a fused variant from the naturally split gp41‐1 intein, ...
Split-intein-mediated protein trans-splicing is predestined for such endeavors and has seen some successes, but a much larger variety of established split inteins and associated protocols is urgently needed. We characterized the association and splicing parameters of the Gp41-1 split intein, which ...
Significance statement: This manuscript focuses on the use of Ultra‐Fast Split Gp41‐1 intein to obtain a fluorescent eukaryotic protein, Maspin, a tumor suppressor protein, and to analyze its cellular localization. This method is based on protein recombination, using split intein and Re...
We characterized the association and splicing parameters of the Gp41split intein, which favorably revealed a nanomolar affinity between the intein fragments combined with the exceptionally fast splicing rate. Following bead﹍oading of a chemically modified intein fragment precursor into live mammalian cells...
Significance statement: This manuscript focuses on the use of Ultra‐Fast Split Gp41‐1 intein to obtain a fluorescent eukaryotic protein, Maspin, a tumor suppressor protein, and to analyze its cellular localization. This method is based on protein recombination, using split intein and Red ...
gp41-1split inteintagless purificationThe current trend in biopharmaceutical drug manufacturing is towards increasing potency and complexity of products such as peptide scaffolds, oligonucleotides and many more. Therefore, a universal affinity purification step is important in order to meet the requirements...