The required transport into the Golgi apparatus of newly synthesized proteins exported from the ER offers a twofold advantage to the cell. First, the rate of movement of membrane and protein through the biosynthetic pathway can be controlled by the selective use of a recycling pathway. Second, ...
The Golgi apparatus is a highly dynamic organelle that changes its shape and architecture concomitantly with the continuous flow and extensive traffic across its compartments. Chemical fixation is too slow to resolve properly most of the rapid shape changes and moreover in multiple cases, obscures th...
Two Undervalued Functions of the Golgi Apparatus: Removal of Excess Ca 2+ and Biosynthesis of Farnesol-Like Sesquiterpenoids, Possibly as Ca 2+ -Pump Agoni... farnesol-like sesquiterpenoids (FLSs) may act as the long sought for but still unidentified agonist (s) for Ca 2+ -pumps in both ...
The Golgi apparatus is responsible for glycolipid biosynthesis and has a critical function in the protein secretory pathway involved in glycosylation, sorting and secretion of newly synthesised proteins received from the endoplasmic reticulum (ER)(Glick and Malhotra, 1998; Glick, 2000; Maccioni et al...
Hackstadt T, Chiramel AI, Hoyt FH, Williamson BN, Dooley CA, Beare PA, de Wit E, Best SM, Fischer ER. Disruption of the Golgi Apparatus and Contribution of the Endoplasmic Reticulum to the SARS-CoV-2 Replication Complex. Viruses. 2021;13:1798. Article CAS PubMed PubMed Central Google...
Munro, S. The Golgin coiled-coil proteins of the Golgi apparatus. Cold Spring Harb. Perspect. Biol. 3, a005256–a005256 (2011). Article PubMed PubMed Central CAS Google Scholar Marra, P. et al. The biogenesis of the Golgi ribbon: the roles of membrane input from the ER and of GM...
In vitro-translated [(35)S]methionine-labeled GAP-43 was incubated with plasma membrane, nuclei, mitochondria, Golgi apparatus and a rough microsome preparation that contained the ER-Golgi intermediate compartment (ERGIC), but not plasma membrane or Golgi apparatus. GAP-43 partitioned into Triton ...
The Rab GTPase Ypt1p and the tethering factors Sec34p and Sec35p, but not Bet3p, a member of the TRAPP complex, were also required for protein sorting upon ER exit. Therefore, the Ypt1p tethering complex couples protein sorting in the ER to vesicle targeting to the Golgi apparatus. ...
The Golgi apparatus (GA) is a key organelle in biosynthetic membrane transport. It is a morphologically complex compartment composed of a central domain of stacked flattened cisternae, flanked at its entry and exit sites by tubular networks with sorting functions, thecis- andtrans-Golgi networks (...
nuclei were segmented using an Otsu thresholding method79. Then nuclei were extended to the border of the cell body using a Voronoi algorithm set to stop when the intensity dropped below a defined threshold. The vesicles composing the Golgi apparatus were identified with an Ostu global thresholding...