Here's an example of how it works: As a ribosome builds an amino acid chain, it's pushed into the endoplasmic reticulum. When the protein is complete, the endoplasmic reticulum passes it along to the receiving side of the Golgi apparatus, called the cis face, which is made up of stacks...
& Ding, J. Structural basis for targeting BIG1 to Golgi apparatus through interaction of its DCB domain with Arl1. J. Mol. Cell Biol. 8, 459–461 (2016). Article CAS PubMed Google Scholar Yang, S. & Rosenwald, A. G. Autophagy in Saccharomyces cerevisiae requires the monomeric GTP-...
Mitochondria are highlighted in yellow and Golgi apparatus in purple. Note the preservation and resolution of membranous and vesicular structures. Scale bars: a = 2 mm; b = 200 µm, c (left) = 1 mm; d, e = 20 µm; f = 2 µm; g = ...
The Golgi apparatus is a central meeting point for the endocytic and exocytic systems in eukaryotic cells, and the organelle’s dysfunction results in human disease. Its characteristic morphology of multiple differentiated compartments organized into stacked flattened cisternae is one of the most recogniza...
The Golgi apparatus is a central meeting point for the endocytic and exocytic systems in eukaryotic cells, and the organelle’s dysfunction results in human disease. Its characteristic morphology of multiple differentiated compartments organized into stacked flattened cisternae is one of the most recogniza...
nuclei were segmented using an Otsu thresholding method79. Then nuclei were extended to the border of the cell body using a Voronoi algorithm set to stop when the intensity dropped below a defined threshold. The vesicles composing the Golgi apparatus were identified with an Ostu global thresholding...
A Predicted WPB size distribution (stars) compared to real data (blue bars), where 1Q-WPBs display instability, α, greater than longer organelles. B HUVECs were fixed and processed to label the Golgi apparatus with an anti-TGN46 antibody and WPBs and cell boundaries as described in Fig....
The mechanisms by which the Golgi apparatus (GA) impacts on cell invasion are poorly understood. The human Golgi Anti-Apoptotic Protein (hGAAP, also known as TMBIM4) is a highly conserved Golgi cation channel that modulates intracellular Ca2+ fluxes. Human GAAP is expressed in all human tissue...
Fig. 3. Determination of redox state of the Golgi apparatus and the endoplasmic reticulum (ER). a) The figures show cells expressing the Golgi- (top) and ER-localized (bottom) ratiometric RoGFP2 redox sensitive probe both in normoxic and hypoxic cells (for more details, see the “Materials...
nuclei were segmented using an Otsu thresholding method79. Then nuclei were extended to the border of the cell body using a Voronoi algorithm set to stop when the intensity dropped below a defined threshold. The vesicles composing the Golgi apparatus were identified with an Ostu global thresholding...