The Golgi apparatus is a crucial organelle responsible for processing and packaging proteins and lipids in eukaryotic cells [1]. However, Golgi proteins themselves play particularly important roles within these functions. These proteins are involved in essential cellular processes such as glycosylation, li...
The Golgi apparatus is a central meeting point for the endocytic and exocytic systems in eukaryotic cells, and the organelle’s dysfunction results in human disease. Its characteristic morphology of multiple differentiated compartments organized into stacked flattened cisternae is one of the most recogniza...
13and the endoplasmic reticulum (ER) and Golgi apparatus compartments play important roles in this process. A major function of the ER is the production and transport of secretory/transmembrane proteins to the Golgi apparatus, from where they are sorted to their final destinations. We previously de...
1. The Golgi apparatus, together apart 2. Golgins: a diverse family of coiled-coil proteins 3. GRASP proteins stack the Golgi together 4. Dynamics of the Golgi matrix 5. Changes in Golgi structure during mitosis and apoptosis 6. Regulation by small GTPases 7. The role of the cytoskeleton...
The markedly greater level of ceramides in ER than in the Golgi apparatus52 indicates that the level of Golgi-localized ceramides reply mainly on the direct supply to the Golgi, rather than on the de novo synthesis of ceramide in the ER. To elucidate the mechanism of starvation-induced ...
The Golgi apparatus is a central meeting point for the endocytic and exocytic systems in eukaryotic cells, and the organelle’s dysfunction results in human disease. Its characteristic morphology of multiple differentiated compartments organized into sta
One approach which has been used to test the role of kinesin in vivo is transfection of cells with a dominant negative mutant of kinesin. No effect on the Golgi complex in mouse fibroblasts was observed at the level of immunofluorescence microscopy [69]; however, it should be pointed out ...
ACBD3. In GFP-expressing cells ACBD3 primarily condensed at the Golgi apparatus (Fig. 2a, upper), which is analogous to normal cells where ACBD3 typically co-localizes with a Golgi-resident protein Golga5 (Fig. S1A). However, in GFP-3A-expressing cells ACBD3 dispersed into the ...
The Golgi apparatus consists of stacks of flattened disc-shaped membrane-bound compartments called cisternae, which are highly concentrated near the peri-centriolar region of the cell. The Golgi complex functions as a molecular factory in which proteins from the endoplasmic reticulum are chemically ...
After 5 min of BFA treatment, Mon2 comple- tely dissociated from Golgi apparatus. However, under the same condition, Golgin97 was still observed on Golgi, indicating the exist- ence of Arl1-GTP on Golgi in the absence of Mon2. Second, we found that depletion of Mon2 didn't abolish or...