An important feature of using SpyTag as the peptide tag for a protein of interest is that SpyTag can then enable a range of subsequent bioconjugation reactions. For example, SpyTag allows irreversible immobilization on surfaces or hydrogels29,30,45, or high-level multimerization on virus-like par...
Interestingly, deletion of BioF homolog CCNA_01647 had no effect on ceramide synthesis despite the fact that the protein has 51% similarity and 35% identity to CcbF (Fig. 2). We note that while the ccna_01647 deletion did not affect ceramide levels, we observed a reduction in MHDAG ...
Interestingly, deletion of BioF homolog CCNA_01647 had no effect on ceramide synthesis despite the fact that the protein has 51% similarity and 35% identity to CcbF (Fig. 2). We note that while the ccna_01647 deletion did not affect ceramide levels, we observed a reduction in MHDAG ...
Further E77X mutations were similarly explored on SpyCatcher2.1. c Positions of mutations screened for SpyDock acceleration, based on Protein Data Bank 4MLI; CnaB2 triad represented in spheres, anchoring sites in yellow, and SpyCatcher2.1 accelerating mutations in green. d S49C resin anchoring ...