卷第17期●论著DCP和Glypican一3在人HCC组织中的表达及临床意义罗红,李惠(1.吉林省四平市中心医院病理科,吉林四平136000;2.吉林省四平市中心医院消化内科,吉林四平136000)摘要:目的研究原发性肝细胞癌(HCC)组织中DCP、Glypican.3的表达情况及其与HCC预后的关系,为其血清学研究提供组织学依据,并为HCC的预后判断提供...
结果:(1)免疫组化检测结果:GPC3在HCC与其他肝组织中的表达差异具有统计学意义(p<0.01)。GPC3对HCC诊断的敏感性和特异性分别为80.7%和99.4%,而AFP仅为37.7%和93.6%,在肝硬化、慢性肝炎、正常肝组织中均未见GPC3和AFP蛋白表达。在早期肝癌中GPC3的阳性表达率为77.2%(44/57),显著高于AFP的35.1%(20/57);并且早...
However, the diagnosis of HCC should not rely entirely on positive GPC3 immunostaining because focal immunoreactivity can be detected in a small subset of cirrhotic nodules. In addition, GPC3 expression in HCC can also be focal, and thus, the lack of ...
目的 探讨glypican-3,HepPar1和CD34在肝细胞癌(HCC)中的表达及临床意义.方法 通过免疫组化方法研究101例肝细胞癌及癌旁组织中glypican-3,HepPar1和CD34的表达情况,探讨其在肝细胞癌诊断中的意义,并分析其与临床病理特征的关系.结果 在HCC中glypican-3阳性率为83.2%,在癌旁正常肝组织内为(-).在HCC... 查看全...
HepPar -1 在肝细胞癌中的表达及意义王 鹏,周新刚,滕晓英,张亮,孙磊,马志园首都医科大学附属北京地坛医院病理科,北京 100015【摘要】 目的 研究肝细胞癌(hepatocellular carcinoma,HCC)、肝脏良性病变组织中精氨酸酶-1(arginase-1,Arg-1)、磷脂酰肌醇蛋白聚糖-3(glypican-3,GPC-3)、肝细胞抗原(Hep-1)的表达...
GPC3-positive HepG2-Red-FLuc HCC cells expressing Luciferase were purchased from PerkinElmer (RRID:CVCL5I98, Bioware, cat. no. BW134280) and were maintained as previously described33. Cells were cultured in a monolayer at 37 °C in Dulbecco’s modified Eagle Medium (DMEM; Gibco) supplemented...
方法通过免疫组化方法研究101例肝细胞癌及癌旁组织中dypican一3、HepParl和CD34的表达情况,探讨其在肝细胞癌诊断中的意义,并分析其与临床病理特征的关系。结果在HCC中glypican-3阳性率为83.2%,在癌旁正常肝组织内为(一)。在HCC中HepParl阳性...文档格式:PDF | 页数:4 | 浏览次数:45 | 上传日期:2016-02-...
We also showed that two humanized anti-GPC3 antibodies (hYP7 and hYP9.1b) in the IgG format induced antibody-dependent cell-mediated cytotoxicity and complement-dependent-cytotoxicity in GPC3-positive cancer cells. The hYP7 antibody was tested and showed inhibition of HCC xenograft tumor growth in...
HCC patients and 146 cases of benign liver lesions were detected by immunohistochemistry.The correlation between clinicopathologicalfeatures and the prognosis was analyzed according to the follow-up material.Results The positive expression rates of Arg-1,GPC-3,Hep-1 in HCC and benign liver lesions ...
Thus the relative specific expressions of GPC3 on cell surface of malignant HCC tissues make it an attractive target for HCC tumor immunotherapy. However, the GPC3-specific T bodies, particularly the GPC3-specific scFv as targeting moieties, remain under development. The present invention addresses ...