therefore ensuring close proximity of the TcdB A domain with its substrate-GTPases. The structure of the central core is similar to that of type Aglycosyltransferases. Type A–familyglycosyltransferasespossess the aspartate—any amino acid—aspartate (D-X-D) motif, which is conserved in all LCGT...
The recombinant RpUGT2 protein, produced heterologously in Escherichia coli, displayed robust catalytic activity towards a spectrum of flavonoids. Mutational analyses revealed that specific amino acid sequence modifications could result in the loss of enzymatic activity in RpUGT2. This study has thus ...
Gene structure and conserved protein motifs of AhUGT family members. A The phylogenetic tree of AhUGTs. B Exon-intron structure of AhUGT genes. Red boxes represent coding sequences, blue boxes are untranslated regions, and black lines indicate introns. C Conserved protein motifs in AhUGTs. Fig...
Identification of alpha-1,2-N-acetylglucosamine (GlcNAc) transferase gene (rfaK) using Tn916; Homology of the amino acid sequence with a family of beta-glycosyltransferases involved in the biosynthesis of polysaccharides.KahlerCharleneM.Carlson
(HMM) for UGT family (Pfam number: PF00201) downloaded from Pfam (version 26.0) (http://pfam.sanger.ac.uk/) was used as queries to search against protein database ofAn. sinensis. Third, the TBLASTN was performed with an E-value cut-off as 1e−5 to search for theAn. sinensis...
Among the various glycosyltransferases (GTs) involved in this process, PglH stands out because it is a membrane-associated enzyme that catalyzes a well-controlled, processive reaction2,7. However, in the absence of a high-resolution structure, it is not known how this B-family GT achieves its...
Glycosyltransferases (GTs) are enzymes that catalyze the formation of glycosidic bonds and hundreds of GTs have been identified so far in humans. Glycosyltransferase 8 domain-containing protein 1 (GLT8D1) has been associated with central nervous system d
UDP-dependent glycosyltransferases (UGTs) be- long to the largest family (family 1) of the glycosyltrans- ferase superfamily and present distinct but overlapping substrate specificities [5]. For the nomenclature system, UGT genes are named using the UGT Nomenclature Committee conventions: the root ...
This finding suggested that α-2,6 sialyltransferase family members may exhibit higher specificity toward antibodies. Methods Cell culture and media for maintenance of CHO K1 master cell line (MCL) The CHO K1 master cell line (MCL) was generated by nucleofection of a landing pad vector into CHO...
It is noteworthy that despite OGT catalyzing a distinct reaction (O-glycosylation), both enzymes are classified in the same CAZy family, GT41, owing to their similarities at the catalytic domain level36. MD simulations for up to 400 ns (Supplementary Fig. 5) were performed to further ...