SARS-CoV-2 survival and virulence are shown to be impacted by glycans, covalently attached to proteins in a process of glycosylation, making glycans an area of interest in SARS-CoV-2 biology and COVID-19 infection. The SARS-CoV-2 uses its highly glycosylated spike (S) glycoproteins to bind...
Predominantly, one glycan structure has been detected per chromatographic peak ranging in their complexity from high-mannose glycans, e.g. M5 in Tf_GP1, to complex tetraantennary sialylated and fucosylated glycans, e.g. F2A4G4S[3,3]2 in Tf_GP33. The most abundant glycans in Tf N-glycome...
Predominantly, one glycan structure has been detected per chromatographic peak ranging in their complexity from high-mannose glycans, e.g. M5 in Tf_GP1, to complex tetraantennary sialylated and fucosylated glycans, e.g. F2A4G4S[3,3]2 in Tf_GP33. The most abundant glycans in Tf N-glycome...
This means that proteoforms with the same masses (within 100 ppm), but different peptide combinations, were sorted based on the better mass match and intensity of the peptides (meaning that closer mass matches and higher intensities do get higher ranks). Next, we discarded all peaks with a ...
All samples that passed calibration and could be matched to their respective maternal or UC partner, meaning that both spectra of a given pair passed calibration, were used for the quantitation. This resulted in 4 pairs of IgG data and 10 pairs of TPNG data being removed, leaving 38 pairs ...
Department of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology, Ministry of Public Health and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, No. 92 Bei’er Road, Heping District, Shenyang, 110001, P. R. China Dianbao Zhang, Xiaoyu ...
Only one of these homologues is incorporated into a given OTase complex, meaning that there exist two isoforms of OTase in yeast, defined by the presence of either Ost3p or Ost6p (Schwarz et al., 2005, Spirig et al., 2005). These different isoforms have been shown to have different ...
(1986) and Krieger et al. Methods in Cell Biology 32: 57-84(1989) have demonstrated that the LDL receptor defect, like other glycoprotein and glycolipid defects in ldlD cells, was “environmentally reversible,” meaning that both glycosylation and function could be restored by the addition of ...
Unless specifically defined herein, all terms used herein have the same meaning as they would to one skilled in the art of this disclosure. Practitioners are particularly directed to Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Press, Plainsview, N.Y....
the origin of eukaryotes from former organisms is one of the most intriguing questions in biology. Endosymbiosis was certainly an important contributor to eukaryogenesis, for at least the mitochondria are known to have evolved from an alpha-proteobacterium that was engulfed prior to the last eukaryot...