By using the HCVcc system, a study showed that an N-glycosylation site in E1 (residue 196) is critical for both E1E2 heterodimerization and viral particles secretion.93 Moreover, several sites have been identified in E2 to be involved for E2 folding and virus entry.94,95 N-Glycosylation ...
In particular, the present invention relates to a vector suitable for transformation of a yeast and comprising an expression cassette comprising a DNA sequence encoding for a viral envelope protein preceeded by the CL leader, or a functionally equivalent thereof. In particular, the present invention...
However, the fact that good fits are achieved with the glycosylated models indicates that the protein structure in the nonglycosylated crystal structure is consistent with that in the fully glycosylated protein. BHA Influenza hemagglutinin is a trimeric viral surface protein that catalyzes host-virus ...
Localization of the UL49.5 protein in the viral envelope was confirmed by immunoelectron microscopy. The treatment of purified virions with glycosidases led to a reduction of the apparent molecular mass in Western blots by approximately 2 kDa following digestion with neuraminidase and O-glycosidase. ...
HIV-2 glycoproteins eluted from the affinity column were resolved by polyacrylamide gel electrophoresis as previously described, and the regions of the gel containing the viral glycoproteins were cut out by reference to the position of prestained molecular weight protein markers (BRL). ...