reuteri 121 IS residues at acceptor subsite − 1, R423K (also interacting with a glucosyl residue at subsite + 1; Arg246 residue in B. subtilis SacB), or W271N (Trp85 residue in B. subtilis SacB), caused altered fructo-oligosaccharide product patterns with sucrose, yielding much less ...
Many drugs cause side effects such as long QT syndrome by blocking the human ether-a-go-go related gene (hERG) K+ channels. To determine whether taxifolin glycoside would block hERG K+ channels, we recorded hERG K+ currents using a whole-cell patch clamp technique. We found that taxifolin...
Many drugs cause side effects such as long QT syndrome by blocking the human ether-a-go-go related gene (hERG) K+ channels. To determine whether taxifolin glycoside would block hERG K+ channels, we recorded hERG K+ currents using a whole-cell patch clamp technique. We found that taxifolin...
The far-UV CD and tryptophan intrinsic fluorescence spectra of all four mutants were essentially identical to those of the WT enzyme (Fig. S8), thus indicating that these mutations did not cause sig- nificant changes in the protein's overall conformation. The K131A mutation had the most ...
C, and an active Both families have a catalytic site with 4 conserved regions r(eβg/aαr)d8ebdaarsreslesqturuenctcuerfeiningetrhperiirntpsrofotreitnhse, 1Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, ...
Many drugs cause side effects such as long QT syndrome by blocking the human ether-a-go-go related gene (hERG) K.+ channels. To determine whether taxifolin glycoside would block hERG K+ channels, we recorded hERG K+ currents using a whole-cell patch clamp technique. We found that ...
The choice of immobilization techn12iqouf 3e5 should be dictated by the enzyme application, since it has been demonstrated that different ipmromtocboiliszlaetaiodntopurontiqocuoelschlaeraadctetroistuicnsi.qTuhee cshearcahctfeorrisntiocvs.el mTahtericseesaarcnhd ifmormnoobvileizl amtioantrsictreast...
Compound Q 7-MeGlu Quercetin T(K) 300 305 310 315 300 305 310 315 Ksv (·104 M−1) 3.837 ± 0.192 * 3.545 ± 0.025 * 3.412 ± 0.045 * 3.182 ± 0.013 * 17.510 ± 0.152 * 16.719 ± 0.135 * 15.632 ± 0.109 * 13.718 ± 0.058 * Kb (·104 M−1) 9.355 ± 1.442 * 5.043 ...