capacity. For the glycolysis stress-test assay, D-glucose (10 mM), oligomycin (1 μM), and 2-deoxy-D-glucose (50 mM) were treated to a cell-culture plate to determine the glycolytic flux, including glycolysis, glycolytic capacity, and glycolytic reserve. Real-time quantitative PCR U...
Hyperoxia decreases glycolytic capacity, glycolytic reserve and oxidative phosp horylation in MLE-12 cells andinhibits complex I and II function, but not complex IV in isolated mouse lung mitochondria. Plos One 8(9).Hyperoxia decreases glycolytic capacity, glycolytic reserve and oxidative phosphorylation...
Terminal T cell exhaustion is characterized by metabolic reprogramming To analyze the transcriptional-metabolic programs along the developmental trajectories of exhausted T cells, we performed single-cell (sc) RNA sequencing of CD44+PD-1hiCD8+T cells isolated from chronically infected wild-type (WT) m...
From these experimental measurements, measures of glycolysis, glycolytic reserve, glycolytic capacity, and non-glycolytic acidification were calculated. ROS staining For DCFDA staining, 100 μL of 40 μM DCFDA (abcam) was added to 100 μL of cells (1x106) and incubated in the dark at 37°C...
C and D Glycolytic rate, capacity and reserve were determined using Seahorse real-time glycolytic stress assay in PF-543-treated (C) or shRNA-mediated SphK1 knockdown (D) primary HUVECs. ECAR, extracellular acidification rate; n = 5. Data are expressed as mean ± SD. *p <...
The recognition that the glycolytic oscillations were not continuously damped came from the observation that the oscillations in cell-free extracts could be sustained by the addition of the reserve disaccharide, trehalose, or by the infusion of glucose, fructose, or phosphorylated hexoses at the ...
For a long time considered a reserve carbohydrate, it gained new importance w... G Carlos,Flores Carmen-Lisset - 《Fems Yeast Research》 被引量: 248发表: 2010年 How proteins get into microbodies (peroxisomes, glyoxysomes, glycosomes) All microbody proteins studies, including one microbody ...
Mycosporine-like amino acids (MAAs) are a class of strongly UV-absorbing compounds produced by cyanobacteria, algae and corals and are promising candidates for natural sunscreen components. Low MAA yields from natural sources, coupled with difficulties in culturing its native producers, have catalyzed ...
Glycogen is referred to as a storage form of glucose; it is an important reserve of fuel. Glycogen is usually stored in the liver and the muscle cells in an animal. There is an alpha-1,4-glycosidic bond between the molecules ...
Finally, the often present coexistence with coronary artery disease leads to a decreased coronary driving pressure beyond the coronary stenosis overstressing the dilator reserve capacity [48]. Thus, in AS, obstruction of the left ventricular outflow tract impairs the systolic and diastolic function of...