Calculation of the ECAR: The maximum glycolytic capacity (max) is the maximum rate of acid production after the addition of oligomycin minus the rate of acid production at time point 3. The basal glycolytic capacity (min) is the maximum rate of acid production after glucose addition minus the...
Along with the data on ECAR, lactate, and glucose consumption, Rev-erbα suppresses glycolytic flux and the PPP in gastric cancer cells by inhibiting PFKFB3 and G6PD gene expression. There are two pathways for Rev-erbα-mediated transcription repression. Once in nucleus, Rev-erbα competes ...
The glial-specific aconitase inhibitor fluorocitrate was used to limit the contribution of glial mitochondria to OCR and ECAR. We observed significant decreases in maximal respiration, ATP production, and spare capacity with aging. In the presence of fluorocitrate, OCR was higher, with more ATP ...
For ECAR (right), a preliminary dose of glucose is used to stimulate extracellular acidification through glycolysis (A; blue), followed by oligomycin treatment to again inhibit ATP synthase and yield maximal glycolytic capacity (B; green). 2-DG is finally used as a glucose analog to ...
In fact, stromal contact resulted in a significantly increased glycolytic capacity (425.9 ± 48.48 vs 355.1 ± 39.66%, baseline ECAR set as 100%) (Figure 1D). Consequently, expression levels of key enzymes controlling glycolysis were significantly higher in those cells including hexokinase-2 (55.71...
(ECAR) and low oxygen consumption rates (OCR), while THP1 cells (MLL-AF9+) and FLT3-ITD-positive AML cells (MOLM13 and MV411) were predominantly OXPHOS-driven (Fig.4a, b). PDK1 protein and mRNA expression were higher in HL60 in comparison to more OXPHOS-dependent THP1 cells (Fig....
A Relative cell viability as measured by alamarBlue® reduction capacity on day 2 and 4 of ATRA treatment. Normalized to untreated control (n = 2, 4 technical replicates each, mean ± SEM). B ATRA titration showing dose-dependent induction of cell death in HK3-null HL60 clone after 4 ...
(J) Overexpression of gLINC in HepG2 cells reversed the retarded ECAR (left) and decreased production of ATP (center) and lactate (right) resulting from c-Myc shRNA knockdown. n = 3, one-way ANOVA. We also further examined the regulatory relationship between c-Myc and gLINC. Measurement ...
C and D Glycolytic rate, capacity and reserve were determined using Seahorse real-time glycolytic stress assay in PF-543-treated (C) or shRNA-mediated SphK1 knockdown (D) primary HUVECs. ECAR, extracellular acidification rate; n = 5. Data are expressed as mean ± SD. *p <...
To explore whether miR-361-5p suppressed glycolysis due to the increased oxidative capacity, we measured the rate of glucose uptake of miR-361-5p BC cells treated with rotenone (an inhibitor of mitochondrial respiration) or etomoxir (an inhibitor of fatty acid oxidation). We observed that ...