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The glycolysis level, glycolytic capacity, and glycolytic reserve of primary TECs were dramatically reduced on PFKFB3 depletion after H/R, whereas no differences were observed under normoxic conditions (Supplementary Figure S8A–D). Similarly, the intracellular lactate level was significantly lower in ...
In fact, stromal contact resulted in a significantly increased glycolytic capacity (425.9 ± 48.48 vs 355.1 ± 39.66%, baseline ECAR set as 100%) (Figure 1D). Consequently, expression levels of key enzymes controlling glycolysis were significantly higher in those cells including hexokinase-2 (55.71...
2b, knocking down the Rev-erbα gene increased basal glycolysis and glycolytic capacity in SGC-7901 cells. Treatment with a Rev-erbα antagonist SR8278 (1 μM and 3 μM) for 48 h significantly increased intracellular levels of lactate in both SGC-7901 and BGC-823 (Fig. 2c). In...
CRISPR/Cas9 mediated gene disruption revealed that loss of HK3 has no effect on glycolytic activity in AML cell lines while knocking out HK2 significantly reduced basal glycolysis and glycolytic capacity. Instead, loss of HK3 but not HK2 led to increased sensitivity to ATRA-induced cell death in ...
The Seahorse Extracellular Flux analysis showed hypoxia enhanced glycolytic capacity of ALKBH5-overexpressing cells, which could be reverted by HDAC4 silencing (Fig. 6B, C). HDAC4 silencing also abolished hypoxia-induced mRNA upregulation of key glycolysis regulatory gene in ALKBH5-overexpressing cells...
ARelative cell viability as measured by alamarBlue®reduction capacity on day 2 and 4 of ATRA treatment. Normalized to untreated control (n = 2, 4 technical replicates each, mean ± SEM).BATRA titration showing dose-dependent induction of cell death inHK3-null HL60 clone after 4 ...
For OCR (left), basal mitochondrial respiration (A; blue) is disrupted with oligomycin treatment to inhibit ATP synthase and demonstrate ATP consumption (B; red), followed by FCCP treatment to uncouple the mitochondria and demonstrate maximal respiratory capacity (C; green). Rotenone treatment to ...
Define glycolytic. glycolytic synonyms, glycolytic pronunciation, glycolytic translation, English dictionary definition of glycolytic. n. A metabolic process that occurs in nearly all living cells in which glucose is converted in a series of steps to pyr
1) Glycolytic capacity 糖酵解效率例句>> 2) glycolytic flux 糖酵解速率3) Glucose glycolysis rate 葡萄糖酶酵解速率4) glycolysis [英][glai'kɔlisis] [美][glaɪ'kɑləsɪs] 糖酵解 1. The unique Entner-Doudoroff (ED) glycolysis pathway of glucose in Archaea——A review; ...