The glycerol phosphate shuttle for transferring cytosolic reducing equivalents to the mitochondria. Cytosolic NADH+H+ is converted to NAD+ by glycerol P dehydrogenase. Glycerol-3-P crosses the outer mitochondrial membrane and is converted back to dihydroxyacetone P by a mitochondrial glycerol-3-P ...
Aerobically, on the other hand, several different redox adjustment mechanisms exist, one of these being the glycerol 3-phosphate (G3P) shuttle. We have studied the importance of this shuttle under aerobic conditions by comparing growth properties and glycerol formation of a wild-type strain ...
G3P shuttle also exists in higher plants [27] and yeasts [28]. In most eukaryotic organisms, the NAD+-dependent GPDH locates in cytoplasm and therefore is also named cytosolic GPDH. The FAD-dependent GPDH exists in mitochondria, so it is also termed mitochondrial GPDH. The two enzymes are...
3-phosphate to DHAP(dihydroxyacetone phosphate) with associated reduction of the enzyme-bound FAD. GPD2 is a testis-specific promoter of mitochondrial GPDH. GPD2 along with a cytosolic NAD-linked GPD forms the glycerol phosphate shuttle that uses the interconversion of G-3-P and DHAP to ...
G3P may enter the G3P shuttle to generate NAD+, or may be converted to glyceraldehyde 3-phosphate and enter glycolysis or the lipid biosynthesis pathway.Glycerol-3-Phosphate Assay Kit has been used in the determination of glycerol-3-phosphate and phosphoenolpyruvate levels. It has also been ...
\ GLYCEROL PHOSPHATE SHUTTLE IN VIRUS-TRANSFORMED CELLS IN CULTURE Mina J. Bissell, Walter A. Rambeck, Rodney C. White, and James A. Bassham July 1975 Prepared for the U. S. Energy Research and Development Administration under Contract W -7405-ENG-48 For Reference Not to be taken from ...
Primers were designed to introduce a Gly5 peptide linker and a ribosome binding site, and to facilitate cloning of the fused gene construct into the Clostridium-Ec shuttle plasmids pWUR459 and pWUR460 at both the 5′ ApaI or NcoI site and the 3′ EcoRI or XhoI site (Fig. 9). Primer...
(26.2-fold increase) gene;ura3gene was analyzed as a positive control (19.2-fold increase). Since all the heterologous genes were cloned under the control of the same G3P dh promoter, the differences in the expression level should not result from differences in a number of transcription ...
G3P shuttle also exists in higher plants [27] and yeasts [28]. In most eukaryotic organisms, the NAD+-dependent GPDH locates in cytoplasm and therefore is also named cytosolic GPDH. The FAD-dependent GPDH exists in mitochondria, so it is also termed mitochondrial GPDH. The two enzymes are...
pDB1334 Vector with cassette ADH1p-Dr_T3-TEF2t Example 1; SEQ ID NO: 3 pDB1336 Vector with cassette PRE3p-Zr_T5-TEF2t Example 1: SEQ ID NO 4 pRN1119 hphMX-bearing shuttle vector based on Example 2; SEQ ID NO: 5 pRS305 CRISPR/Cas9 genome editing was used to perform genetic modifi...