Glycine-NaOH Buffer(甘氨酸-氢氧化钠缓冲液),50mM,pH9.0 邦景品牌 上海邦景实业有限公司 2年 上海 查看详情 ¥240.00元 ≥1千克 N,N-二羟乙基甘氨酸99%含量高纯度生物缓冲剂 CAS:150-25-4 真实性已核验 湖北瑞伯信化工有限公司 1年 湖北襄阳 查看详情 ¥20.00元 ≥1瓶 10xTris-甘氨酸SDS电泳缓...
Yes. You can use a 15% denaturing polyacrylamide gel for Northern analysis of small RNAs, such as miRNAs and siRNAs. A hyridization buffer optimized for use with short probes, such as ULTRAhyb-Oligo solution, should be used for the best results. For more sensitive detection, enrich the RNA...
Incubate the membrane with 400 mM NaOH for 30 minutes, then wash with 0.1% SDS for 15 minutes. These stripping methods should work for 2 to 3 stripping procedures. However, nucleic acids will gradually be removed from the blot. Poor signal could be a result of the following: - A ...
After removing methanol, the pellet was suspended with 143 μl of ultrapure water, and mixed with 7.5 μl of 5 N NaOH and boiled for 5 min. The α-glucan sample was desalted and subjected to hydrolysis with isoamylase (0.03 U/mg of amylopectin in 40 mM acetate buffer, pH 4.4) at ...
Frozen cell pellets (107 cells) of mock or ST3GAL4 transfected MKN45 cells were directly resuspended in 2 mL of lysis buffer (50 mM Tris–HCl, 100 mM NaCl, 1 mM EDTA and protease inhibitor at pH 7.4) and stored on ice for 20 min. The cells were lysed using a Polytron homogenizer ...
(pH8.O):EDTA-Na2’2H2033.629双蒸水 150ralNaOH49调节pH值至8.0,定容至200ml,高压灭菌2.1MTri-HCI(pH8.01:双蒸水800mlTris碱121.19浓盐酸调节pH8.0定容1L,分装后高压灭菌3.TE缓冲液:1MTri—HCl0H7.4)1.0ml0.5MEDTAOH8.o)20pllO上海试剂三厂产品美国MJ公司产品常州国华电器有限公司上海精密科学仪器有限公司...
IMAC purified fusion toxin was bound to the column in 27 mM KH2PO4, 46 mM NaOH, 10 mM EDTA, 0.75 M NaCl, 0.1% Tween 20, pH 8, and after washing in the same buffer, elution was performed in 0.1 M Na2CO3, 0.5 M NaCl, 0.1% Tween 20, at pH 11.3. The eluted sample was ...
(NaOH) [7,35,39]. However, the newly created reducing end of the released glycan is sensitive to base, which can cause degradation through the “peeling reaction,” a repetitive β-elimination type reaction that can deconstruct the oligosaccharide from its reducing end. This degradation is ...
Reaction was stopped by adding 0.2 M NaOH (20 µL). 3.6. HPLC-Separation DMB-labeled samples were analyzed on a Superspher 100 C-18 column (250 mm × 40 mm, Merck-Hitachi, Darmstadt, Germany) at 40 °C...Galuska SP, Geyer H, Mink W, Kaese P, Kuhnhardt S, Schafer B, Muh...
Die Erfindung betrifft ein isoliertes Polynukleotid mit Promotoraktivität, eine Variante des Promotors des für die Glyzerinaldehyd-3-phosphat-Dehydrogenase kodierenden gap-Gens; sowie einen Mikroorganismus, der eine Feinchemikalie produziert und/oder ausscheidet, wobei der Mikroorganismus das ...