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At high pH, the hydroxyl groups of sugars become ionized, and the resulting negative charge can be exploited for separation on an anion exchange resin [21]. Neutral and amino sugars are eluted isocratically with 16 mM NaOH, and acidic sugars, such as uronic and sialic acids, are eluted with...
(e.g., D-gluconic acid oxidized from D-glucose) and uronic acid which is carboxylic acid derived from a terminal C atom (D-glucuronic acid oxidized from D-glucose)), sugars having an amino group or an amino group derivative (e.g., an acetylated amino group) (e.g., N-acetyl-D-...
However, what is known is that increased levels of sialic acid can affect absorption, serum half-life and clearance from the serum, as well as the physical, chemical and immunogenic properties of various key serum glycoproteins (Alavi and Axford, 2008; Bork et al., 2009; Schauer, 2009). ...