Application ELISA Detection Method Colorimetric Shipping Condition Blue ice Storage Instructions -20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. ...
Kit Summary: • Detection method- Absorbance (340 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum,erythrocyte lysates, as well as many other biological fluids etc. • Species reactivity- Mammalian • Application- The assay has adetection sensitivity ...
Cellular apoptosis was assessed with an Annexin V-FITC apoptosis detection kit (Beyotime) according to the manufacturer’s instructions. In brief, 143B cells were seeded on 12-well plates at 3 × 105 cells per well. After 12 h incubation, cells were treated with PBS, Ru(II)-OH, ...
Reverse transcription was done using the RevertAid first-strand cDNA synthesis Kit (Thermo Fisher Scientific, USA) and quantitative PCR was performed using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA) on a Bio-Rad CFX96-Real-Time PCR Detection System. Relative expression levels ...
Application of glutathione depletion in cancer therapy: enhanced ROS-based therapy, ferroptosis, and chemotherapy. Biomaterials. 2021;277: 121110. Article PubMed CAS Google Scholar Stockwell BR, Angeli JPF, Bayir H, Bush AI, Conrad M, Dixon SJ, Fulda S, Gascón S, Hatzios SK, Kagan VE, ...
2.6. Detection of carbonylated proteins Carbonylated proteins were detected and analyzed after the derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (Protein Oxidation Detection Kit, OxyBlot™, Millipore). Derivatized samples were incubated in Laemmli sample buffer for 5 min at ...
An RNAqueous kit (Ambion) was used to isolate the total RNA from the different culture combinations, which included the explants that had been induced on the E0, E5 and E0 media with 0.5 mM GSH (E0G). The explants that had been induced on the E0G medium that were capable of SE induct...
& Qu, B. A disulfide bound-molecular beacon as a fluorescent probe for the detection of reduced glutathione and its application in cells. Chem. Commun. 48, 3221 (2012). 11. Deng, R., Xie, X., Vendrell, M., Chang, Y. T. & Liu, X. Intracellular Glutathione Detection Using MnO2-...
16. The kit according to claim 15, wherein the compound containing an —SH group is a compound having a cysteine residue. 17. The kit according to claim 15, wherein the compound containing an —SH group is glutathione.Description: This application is a National Stage of International Patent...
Application Ser. No. 61/379,203 which was filed Sep. 1, 2010. SUMMARY OF THE INVENTION The present invention provides an assay for detection of oxidized glutathione (GSSG) and the determination of the ratio of GSH and GSSG in a cell. BACKGROUND OF THE INVENTION To remain healthy, cells,...