The GSH detection assay is based on the conversion of a luciferin derivative into luciferin in the presence of GSH. The reaction is catalyzed by a glutathione S-transferase (GST) enzyme supplied in the kit. The luciferin formed is detected in a coupled reaction using Ultra-Glo™ Recombinant ...
Application of glutathione depletion in cancer therapy: enhanced ROS-based therapy, ferroptosis, and chemotherapy. Biomaterials. 2021;277: 121110. Article PubMed CAS Google Scholar Stockwell BR, Angeli JPF, Bayir H, Bush AI, Conrad M, Dixon SJ, Fulda S, Gascón S, Hatzios SK, Kagan VE, ...
Cellular apoptosis was assessed with an Annexin V-FITC apoptosis detection kit (Beyotime) according to the manufacturer’s instructions. In brief, 143B cells were seeded on 12-well plates at 3 × 105 cells per well. After 12 h incubation, cells were treated with PBS, Ru(II)-OH, ...
Kit Summary: • Detection method- Absorbance (405 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum,erythrocyte lysates, as well as many other biological fluids etc. • Species reactivity- Mammalian • Application- The assay can detect 0.1 – 40 mU...
2.6. Detection of carbonylated proteins Carbonylated proteins were detected and analyzed after the derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (Protein Oxidation Detection Kit, OxyBlot™, Millipore). Derivatized samples were incubated in Laemmli sample buffer for 5 min at ...
A major problem associated with the use of BH3 mimetics is the lack of specific biomarkers that can guide their application. Several genetic lesions have been identified to predict the efficacy of Venetoclax, including mutations inIDH1/2andSRSF2/ZRSR269,70. Recent studies proposed that high expre...
Among these, theaspirane has been detected in peach and pear juice, but only at concentrations below the odour detection threshold (i.e. the lowest concentration perceivable by the human sense of smell) (Niu et al., 2021). Conversely, the wine samples all had theaspirane concentrations ...
NMR-based metabolomics: translational application and treatment of cancer. Curr Opin Mol Ther 2007; 9: 572–585. CAS PubMed Google Scholar Tian Y, Nie X, Xu S et al. Integrative metabonomics as potential method for diagnosis of thyroid malignancy. Sci Rep 2015; 5: 14869. Article CAS ...
Application of NEM rapidly alkylated cellular thiols, indicated by the stabilization of reduced PRDX3 within 30 s of treatment (Supplementary Fig. 6a). Importantly, this did not interfere with the capacity to isolate mitochondria from HA-Mito cells (Supplementary Fig. 6b). Fig. 5: CHAC1 ...
Lou, X., Hong, Y., Chen, S.et al.A Selective Glutathione Probe based on AIE Fluorogen and its Application in Enzymatic Activity Assay.Sci Rep4, 4272 (2014). https://doi.org/10.1038/srep04272 Download citation Received26 November 2013 ...