Synechococcus glutamate-1-semialdehyde aminotransferase was expressed in large amounts in transformed cells of Escherichia coli. The resulting purified enzyme has an absorption spectrum characteristic of B6-containing enzymes and could be converted to the pyridoxal-phosphate form with excess dioxovalerate (...
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The compounds serve as inhibitors for pyridoxal 5′-phosphate-dependent (2S)-glutamate 1-semialdehyde aminotransferase, a target for selective herbicides and antibacterial agents on the tetrapyrrole biosynthetic pathway. The (2R)-enantiomer, as predicted, serves as a potent irreversible inactivator....
Structural genes of glutamate 1-semialdehyde aminotransferase for porphyrin synthesis in cyanobacterium and Escherichia coli. Mol. Gen. Genet. 225, 1-10Grimm, B., Bull, A., and Breu, V., Structural genes of glutamate 1-semialdehyde aminotransferase for porphyrin synthesis in a Cyanobacterium and ...
GSA aminotransferases containing either the deletion or the substitution that are characteristic of the GR6 mutant were produced in using the expression vector pMalc2. These engineered mutant enzymes were characterized in terms of their catalytic parameters and sensitivities to gabaculine and AFPA. ...
G. 1990 . The glutamate I -semialdehyde aminotransferase of barley: purification of the protein. cDNA cloning, sequencing and characterization of the mRNA .—In: Currenr Research in Photosynthesis Vol. III (ed. Baltscheffsky ), Kluwer Acad. Publ., Netherlands ,p. 585 – 587...
Grimm B, Smith AJ, Kannangara CG, Smith M. Gabaculine-resistant glutamate 1-semialdehyde aminotransferase of Synechococcus. Deletion of a tripeptide close to the NH2 terminus and internal amino acid substitution. J Biol Chem. 1991 Jul 5; 266 (19):12495–12501....
Gough KC,Allison G,Rogers L, et al.In vitro characterization of the inhibitor resistance ofglutamate-1-semialdheyde aminotransferase from the cyanobacterium Synechococcus PCC6301GR6. European Journal of Phycology . 2001In vitro characterization of the inhibitor resistance ofglutamate-1-semialdheyde ...
In vitro selectionGabaculineTransgenic plantsTransplastomic plantsNew selection systems are required to extend plastid transformation to a more significant number of plant species. After demonstrating that a bacterial mutant glutamate 1-semialdehyde aminotransferase (GSA) gene is functional in tobacco ...
(2005) Cellular levels of glutamyl-tRNA reductase and glutamate-1-semialdehyde aminotransferase do not control chlorophyll synthesis in Chlamydomonas reinhardtii. Plant Physiol 139, 389-396.Nogaj, L. A. & Beale, S. I. Physical and kinetic interactions between glutamyl-tRNA reductase and glutamate-1...