The glutamate concentrations in mitochondrial and cytoplasmic fractions were measured using a glutamate assay kit (Sigma, USA). The assay was based on enzymatic conversion, and the absorbance was read at 450 nm using a microplate reader (FlexStation3, Molecular Devices, USA). Then, glutamate pro...
The fluorescent glutamate indicator iGluSnFR enables imaging of neurotransmission with genetic and molecular specificity. However, existing iGluSnFR variants exhibit low in vivo signal-to-noise ratios, saturating activation kinetics and exclusion from postsynaptic densities. Using a multiassay screen in bac...
The cell viabilities in vehicle and various concentrations of glutamate treated cells were assessed using MTT assay kit after 24 h of incubation (Fig. 1a). The results showed that incubation with 2 mM of glutamate reduced the cell viability from 98.8 ± 1.2% in control to 94.2 ± 0.6% (p...
Pierce™ BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Experimental models: Organisms/strains ΔNp73-Cre-IRES-GFP Dr Fadel Tissir, UC Louvain, Belgium Tissir et al. (2009) B6.FVB(Cg)-Tg(Pde1c-cre)IT146Gsat/Mmucd MMRRC stock number: 036699-UCD; RRID:MMRRC_036699-UCD B6;...
However, glutamate- and BDNF-induced enhancements of translation in axons exhibit different kinetics. Moreover, Ca2+ and mTOR signaling appear to play roles carrying different weights, respectively, in transducing glutamate- and BDNF-induced enhancements of axonal translation. Thus, our results indicate...
Increases during aging in extracellular levels of glutamate (Glu), the major excitatory neurotransmitter in the brain, may be linked to chronic neurodegenerative diseases. Little is known about the molecular responses of neurons to chronic, moderate incr
Protein concentration was measured with a BCA Protein Assay Kit (Pierce). Clarified cell extracts were mixed with 6× sodium dodecyl sulfate (SDS) sample buffer. Protein samples were run on 8% SDS-polyacrylamide gel electrophoresis using a Bio-Rad gel system and transferred onto nitrocellulose ...
(Extended Data Fig.6b,c). The three-dimensional structural proximity of Lys380 and Lys392 rationalized these two residues orientated towards the same binding pocket. The Arg160 mutant was not pursued further because it generated very little currents with markedly altered kinetics, making it ...
Optimal precision in achieving concentrations of drug within the range that yields efficacy without toxicity requires a regimen based on the kinetics of the drug's availability to target sites. This involves a consideration of the distribution, equilibrium, and elimination of a drug. The following ...
Optimal precision in achieving concentrations of drug within the range that yields efficacy without toxicity requires a regimen based on the kinetics of the drug's availability to target sites. This involves a consideration of the distribution, equilibrium, and elimination of a drug. ...