A series of solid acid catalysts including SO 4 2/ZrO 2, SO 4 2/TiO 2, SO 4 2/ZrO 2–TiO 2 and SO 4 2/ZrO 2–Al 2O 3 were prepared by precipitation and impregnation method for ethyl levulinate production from glucose in ethanol. Effects of various reaction parameters and catalyst...
Protein purification and transcriptional studies show that two possible candidates can contribute to the first reaction. Most of the enzyme activity in the cultured cells could be accounted for by a protein with an M r of 43kDa which had dual specificity for UDP-glucose and ethanol. The cognate...
The catalytic efficiency of mixed Cu(I)–Cu(II) system in situ generated by partial reduction of CuSO4 with glucose in ethanol (nonanhydrous) under open air has been explored. With this catalysis, the multicomponent cascade reaction of A3-coupling of heterocyclic amidine with aldehyde and alkyne...
In a next step, Glc1P was precipitated with ethanol but this did not induce crystallization, in contrast to what is observed with other glycosylphosphates. After conversion of the product to its cyclohexylammonium salt, however, crystals could be readily obtained. Although both purification methods ...
(PI) were confirmed by reverse transcriptase polymerase chain reaction (RT-PCR; Fig.4A,E). The fact that islets and pseudoislets have similar amounts of insulin mRNA facilitates a comparison between the two with regard to the expression of other β-cell-specific genes. The mRNA levels of ...
This compound is sparingly soluble in water but soluble in organic solvents such as ethanol and acetone. It is important to handle alpha-D-Glucose pentaacetate with caution as it may cause irritation to the skin, eyes, and respiratory system. Ingestion or inhalation of this compound may also ...
estimated from activities of NADHferricyanideand decreased activity of G-6PDH. The changes inhepatic enzymeswith ethanol treatment were paralleled with those of intestinal ones, indicating that metabolic changes in the intestine contribute in some way to the formation of alcoholicfatty liver(Ohta...
β-glucosidases are important enzymes that catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production. Due to this important role, many groups around the world are focusing on the field of Bgls for sev...
Quantitative real-time polymerase chain reaction (qPCR) The primers used were listed in Supplementary Table S3. The isolation of total RNA, the transcription of RNA to cDNA, and the performance of qRT-PCR with Applied Biosystems 7300HT machine were all according to our previous study42. The β...
3-BD synthesis in acetate elimination may provide a particular component required for the AdhE-catalyzed reaction. Since AdhE catalyzes NADH-mediated conversion of acetyl-CoA to ethanol, effects of 2,3-BD synthesis and ethanol production on the NADH/NAD+ratio were assayed. As shown in Fig.2d,...