(FBS) levels were measured in all animals weekly using a glucometer (Abbott Diabetes Care). Finally, all rats were sacrificed by cervical dislocation following anesthesia with an intraperitoneal injection of sodium pentobarbital (45 mg/kg; MilliporeSigma) to reduce pain. The following samples were ...
Blood glucose was measured at 0 (immediately before glucose injection), 15, 30, 60 and 120 min with a Freestyle Lite glucometer (Abbot Diabetes Care). Food intake monitoring. Mice were housed individually. Food weights were recorded every other day over a period of 1 week (four individual ...
Functional analyses were performed by assembling the non-host reads into contigs using MEGAHIT v1.1.2 [36] and mapping those reads against the contigs with bowtie2. Reads that did not assemble were appended to the contigs. Next, Prodigal v2.6.342 [37] was used for predicting codifying regio...
Libraries were sequenced on an Illumina HiSeq 2000 machine. The quality of the sequenced libraries was assessed using fastQC. Reads were mapped to the Grc38m assembly using HiSat2. Annotated transcripts were quantified using featureCounts, and differentially expressed genes were identified with DESeq...
Four samples were pooled per lane and sequenced using paired end 100 bp Illumina Hiseq2000. Processing of raw data and analyses were performed as for mouse RNAseq except that reads were aligned with human transcriptome. For 16S rRNA gene sequencing, complementary DNA was prepared from RNA ...
Glucose and lipid metabolism are crucial functional systems in eukaryotes. A large number of experimental studies both in animal models and humans have shown that long non-coding RNAs (lncRNAs) play an important role in glucose and lipid metabolism. Prev
ere demolded and the thickness was measured using a micrometer screw gauge (Mitutoyo, Japan). 2.2. Fabrication of the PBA-Based HEMA Contact Lens 2.2. FabTrhiceatfiaobnroicf athtieoPnBoAf-BthaesedPBHAE-MbaAseCdonHtaEcMt LAenscontact lens followed three steps: (i) synthesis of HETMhAe-Of...
The ratiometric Ca2+ measurement was performed with Fura-2 AM using a spectrofluo- rometer (SpectraMax® Gemini™ EM, Molecular Devices) and following the manufacturer's instructions. The fluorescence ratio (340/380 nm) was used to monitor changes in cytoso- lic Ca2+ after the TCDD ...
(wleeiftrthe) vathneerdsible swellirnevgebrseihbalevsiowreullpinognbpehHavcihoarnugpeobneptHwecehnanpgHe b5eatwndeepnHpH105aasnmd peHasu10reads mbyeaDsuLrSed(lebfyt)DaLnSd(ltehfet)dainadmeter change of PBA-NPs upon the addition of D-glucose (triangle) and D-fructose (circle) as ...
Development of obesity-associated comorbidities is related to chronic inflammation, which has been linked to gut microbiota dysbiosis. Thus, modulating gut microbiota composition could have positive effects for metabolic disorders, supporting the use of