Blood glucose was measured through tail vein bleeding using the OneTouch UltraVue automatic Glucometer (LifeScan). For measuring insulin levels, approximately 100 μL of blood was collected (from the submandibular vein plexus) and was placed at room temperature for 20 min, followed by centrif...
Glucose was measured in freely moving unrestrained animals using a glucometer (AccuCheck Mobile, Roche) in a drop of blood obtained after incision of the tip of the tail. 2.6. In vivo tissue insulin response measurement Mice were fasted overnight then injected with insulin (0.75 U/Kg, ...
Weight (at the beginning and end) and height (at the beginning) were determined by using a digital scale and stadiometer (Seca® Germany, Model: 7551021994). Body mass index (BMI) was calculated via dividing weight in kilograms by squared height in meters. ...