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Cells were (1.0 × 105) were incubated overnight on 6-well plates, intracellular lactate and ATP production were determined with Lactate Colorimetric/Fluorometric Assay Kit (Biovision, Abcam) and ATP Bioluminescence Assay Kit CLS II (Roche Applied Science) according to the manufacturer’s pro...
Free fatty acids (FFAs) were tested using a quantification colorimetric kit (BioVision, USA). Plasma insulin (Crystal Chem, USA), adiponectin (Millipore, USA), leptin (Crystal Chem, USA), LBP (Abnova, Taiwan China), L-1b, IL-6, MCP-1, TNF-α, glucagon-like peptide-1 (GLP-1) (...
Lactate level PFK-1 kinase activities were measured using colorimetric assay kit according to the manufacturer's protocol (Biovision). The results are expressed as the ratios of experiment to control from three independent experiments. The oxygen consumption rates (OCRs) were measured using the ...
NAD+/NADH Quantification Colorimetric Kit was purchased from Biovision (K337-100, USA). EasyPure RNA Kit (used for RNA extraction) and TransStart Top Green qPCR SuperMix (used for qPCR) were purchased from Transgen (Beijing, China). HiScript II Q RT SuperMix for qPCR (+gDNA wiper, used ...
HK and PK activity assay. HK and PK activity in cells treated with Chaetocin in the presence and absence of SP600125 for 12 h was measured with Hexokinase Colorimetric Assay Kit (Biovision Inc., Milpitas, CA, USA) and PK Activity Assay Kit (Sigma), respectively, according to the ...
Heterozygous HNF1A gene mutations can cause maturity onset diabetes of the young 3 (MODY3), characterized by insulin secretion defects. However, specific mechanisms of MODY3 in humans remain unclear due to lack of access to diseased human pancreatic cell
Pyruvate colorimetric assay kit (K676-100, Biovision) was applied for measurement of lactate production. The supernatant of cells was collected for measurement of lactate production, which was examined at 450 nm with a microplate reader.
We used a citrate synthase activity colorimetric assay kit (Cat # 318-100, BioVision), and followed the manufacturer’s instructions. We homogenized BAT (~10 mg) with 100 μl of the provided citrate synthase assay buffer, we centrifuged the homogenate for 5 min at 10,000 × g...
Levels of NADH, NADPH and OAA in the islets or MIN6 cells were measured with PicoProbe NADH Fluorometric Assay Kit (BioVision), PicoProbe NADPH Quantitation Fluorometric Assay Kit (BioVision) and Oxaloacetate Colorimetric/Fluorometric Assay Kit (BioVision), respectively, according to the manufacturer...