Inclusion complexation of tetrathiafulvalene in cyclodextrins and bioelectroanalysis of the glucose-glucose oxidase reaction - ScienceDirectTetrathiafulvalene (TTF) forms water-soluble inclusion complexes with α-, hp-β- and γ-cyclodextrin (CD). Cyclic voltammetry (CV) has been performed at a ...
Interference is controlled by using a blank determination which has not been treated with glucose oxidase. The reaction has been used to estimate glucose levels in Saccharomyces cerevisiae fermentation and -amylase, invertase and β-galactosidase reactions and coloured corn-steep fermentation media....
Glucose oxidase and catalase were immobilized on poly(diethylaminoethyl methacrylate-g-ethylene glycol) gels by copolymerization of the constituent monomers and the functionalized enzyme solutions. The hydrogels were prepared in the form of discs and microparticles. The amount and the activity of enzymes...
Colorimetric estimation of d-glucose and 2-deoxy- d-glucose with glucose oxidase A modification of Keston's glucose oxidase method for d-glucose analysis is described. The glucose oxidase-peroxidase reaction was carried out in phosphate buffer, pH 5.9 at 45掳, for 30 minutes. A clear stable ...
(1989). Electrochemical reaction of glucose oxidase at graphite electrodes. Bioelectrochem. Bioenerg., 21, 133-148.A. Szucs, G. D. Hitchens, and J. O’M. Bockris, J. Electroanal. Chem. 275 , 133 (1989).Szucs, A., Hitchens, G.D., Bochris, JO'M., 1989. Electrochemical reactions ...
展开 关键词: GLUCOSE OXIDASE DISCOVERY SOURCES PHYSICAL PROPERTIES STRUCTURE STABILITY INHIBITORS FAD SUBSTRATES REACTION MECHANISM BIOSENSOR ENZYME ELECTRODE GLUCOSE SENSOR ELECTROCHEMICAL MEDIATORS DOI: 10.1016/0956-5663(92)87013-F 被引量: 1147 年份: 1992 收藏...
Glucose oxidase in presence of glucose (substrate) produces hydrogen peroxide (H2O2). When such an enzymatic reaction is allowed to occur in presence of rat erythrocytes (in physiological saline) pre-incubated with sodium azide, the erythrocytes are lysed. The extent of erythrocyte lysis is estimate...
Glutathione appears to have a role in protecting cells against cytotoxicity attributed to spermine-derived aldehyde(s), most likely by conjugation in a reaction catalyzed by glutathione S-transferase, whereas metabolism of glucose via the pentose cycle did not. The metabolism of both glucose and ...
The benzoquinone can be used for spectrophotometric measurement of glucose oxidase activity. Reaction mixtures containing 1 M glucose, 0.1% benzoquinone, and 0.1 M Na-citrate buffer, pH 5.0 were preincubated at 25 °C and the reaction was initiated by adding the enzyme solution. The method was ...
This approach involves an enzyme–substrate reaction that results in a pH change and a pH-sensitive polymer that responds to the change. Glucose reacts with glucose oxidase (GOD) forming gluconic acid (GlucA) and, thus decreasing the pH of the environment. With the change in pH, the gel ...