This occurs with a K m of 20 mM, 100 times the corresponding value for pyruvate, and a V max approximating 1/710 that of KDPG cleavage. Ketobutyrate is competitive with both pyruvate and 2-keto-3-deoxygluconate-6P for the enzyme. In addition, there is no evidence for C-C synthesis ...
2-Keto-3-deoxygluconate-6P (KDPG) aldolase ofPseudomonas putida mediates the cleavage of, as well as the condensation of, pyruvate andd-glyceraldehyde-3P (GaP) yielding, 2-keto-3-deoxygalactonate-6P (KDPGal) as side reactions of normal catalysis. These are visualized at high levels of ...