Physiological compensation in antisense transformants: specific induction of an "ersatz" glucan endo-1,3-beta-glucosidase in plants infected with necrotizi... RS Beffa,JM Neuhaus,F Meins - 《Proceedings of the National Academy of Sciences》 被引量: 127发表: 1993年 Physiological compensation in ...
indica. No barley endo-1,3-b-glucosidases were detected at 5 dpi. Among the most prevalent plant proteins present at 10 and 14 dpi we could detect two and four barley endo-1,3-b-glucosidases, respectively (Supplementary Table 1). This correlates with the expression pattern of FGB1 ...
In particular, endo-type β-(1→3)-glucanases are classified into the enzyme commission (EC) number EC 3.2.1.6 endo-1,3(4)-β-glucanase; EC 3.2.1.39 represents glucan endo-1,3-β-D-glucosidase; and EC 3.2.1.73 indicates licheninase. Our results showed that VvGH17 was classified into...
Further insight into the structure of marennine was provided by the spectrum of 1H NMR, MS, a colorimetric assay, and a computational study, which suggest that the chemical structure of marennine contains 1,3-β-glucan. Keywords: marennine; Haslea ostrearia; endo-1,3-β-glucanase; β-...
The membrane-integrated synthase FKS is involved in the biosynthesis of β-1,3-glucan, the core component of the fungal cell wall1,2. FKS is the target of widely prescribed antifungal drugs, including echinocandin and ibrexafungerp3,4. Unfortunately, the mechanism of action of FKS remains eni...
In this assay, highly purified endo-1,3(4)-β-glucanase (lichenase) and β-glucosidase are employed. The glucan is depolymerized by lichenase to oligosaccharides, these oligosaccharides are quantitatively hydrolyzed by β-glucosidase to glucose, and this is specifically measured using glucose ...
A very good example of beta-glucan catabolic enzymes is the Family 3 ofglycosyl hydrolases(GH3), which consists of nearly 44 beta-glucosidases andhexosaminidasesfrom bacteria, molds, and yeasts. Most of the fungal beta-glucosidases studied belong to GH3. Structural data on representatives of GH3...
Comparison of cloned genes provides evidence for intergenomic exchange of DNA in the evolution of a tobacco glucan endo-1,3-beta-glucosidase gene family. Glucan 1,3-beta-GlucosidaseLectinsCloning, MolecularProtein ConformationSequence Homology, Amino AcidAmino Acid SequenceThe prophenoloxidase activating ...
The composition of plant cell walls is important in determining cereal end uses. Unlike other widely consumed cereal grains barley is comparatively rich in (1,3;1,4)-β-glucan, a source of dietary fibre. Previous work showed Cellulose synthase-like genes
1,* 1 Department of Food Science and Engineering, Ningbo University, Ningbo 315832, China 2 Department of Food Science and Technology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA * Author to whom correspondence should be addressed. ...