Fifty μL of protein extracts from germinated grains were incubated with 50 µL of HR solution (containing pNPG7 substrate and α-glucosidase) for 10 min at 40 °C. The incubation was stopped by adding 0.1 mL of 1 M sodium carbonate solution in an Elisa plate and optical density was...
The membrane-integrated synthase FKS is involved in the biosynthesis of β-1,3-glucan, the core component of the fungal cell wall1,2. FKS is the target of widely prescribed antifungal drugs, including echinocandin and ibrexafungerp3,4. Unfortunately, the mechanism of action of FKS remains eni...
indica. No barley endo-1,3-b-glucosidases were detected at 5 dpi. Among the most prevalent plant proteins present at 10 and 14 dpi we could detect two and four barley endo-1,3-b-glucosidases, respectively (Supplementary Table 1). This correlates with the expression pattern of FGB1 ...
A very good example of beta-glucan catabolic enzymes is the Family 3 ofglycosyl hydrolases(GH3), which consists of nearly 44 beta-glucosidases andhexosaminidasesfrom bacteria, molds, and yeasts. Most of the fungal beta-glucosidases studied belong to GH3. Structural data on representatives of GH3...
The composition of plant cell walls is important in determining cereal end uses. Unlike other widely consumed cereal grains barley is comparatively rich in (1,3;1,4)-β-glucan, a source of dietary fibre. Previous work showed Cellulose synthase-like genes
1,* 1 Department of Food Science and Engineering, Ningbo University, Ningbo 315832, China 2 Department of Food Science and Technology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA * Author to whom correspondence should be addressed. ...
Further insight into the structure of marennine was provided by the spectrum of 1H NMR, MS, a colorimetric assay, and a computational study, which suggest that the chemical structure of marennine contains 1,3-β-glucan. Keywords: marennine; Haslea ostrearia; endo-1,3-β-glucanase; β-...