Fluorescence titration studies found that the binary ensemble displays selective turn-off responses to the acidic amino acids Asp and Glu. The detection limits for Asp and Glu were determined to be 0.6 and 2.1 渭M, respectively. Time-resolved decays revealed that the quenching by Asp and Glu ...
High sensitive detection was obtained by the LIF detection. The LODs (S/N = 3) for D -Asp and D -Glu were 6.0×10–8 and 4.0×10–8 M, respectively. Using this method, the levels of D -Asp and D -Glu in rat brain and human cerebrospinal fluid (CSF) were determined.doi:10.1002...
Driving force for separating peptides by HILIC comprises (i) insufficient retention in RPLC, (ii) orthogonal selectivities to RPLC, (iii) enhanced sensitivity in ESI-MS detection (better ionization efficiency at high organic), and (iv) particular retention patterns, e.g., for glycopeptides (vi...
Ac-DEVD-AMC is a fluorogenic substrate that can be cleaved specifically by caspase-3.{13020} Caspase activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm. ...
Glutamic Acid (Glu) has a negativly charged side chain and is soluble. We offer Fmoc-D-Glu(OtBu)-OH, Fmoc-D-Glu-OtBu, and Fmoc-Glu(OtBu)-OH.FiltersProducts Labeling & detection Assay Kits Peptides Proteins Reagents for peptide synthesis ...
Caspase activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm. 序列 Ac-DEVD-AMC 纯度 ≥95% 储存/保存方法 Store at -20℃,1 year. 基本信息 别名 AC-DEVD-AMC 外观 white ...
谷氨酸-谷氨酸标签小鼠单克隆抗体Glu-Glu, 【中文名称】:谷氨酸-谷氨酸 标签 小鼠单克隆抗体(2G1) 【英语名称】:Glu-Glu Tag Mouse Monoclonal Antibody(2G1) 【产品类型】:标签抗体 【供应商】:Abbkine 【产品货号】:ABM40390 【产品
The detection solution was then added, and the membrane was exposed to film for 5 min, after which the protein was visualized (ECL: enhanced chemiluminescence Plus Western blotting detection system; Amersham Bioscience, United Kingdom). The relative amounts of UGT1A1 expressed at the protein ...
Simple and rapid enzyme‐linked immunosorbent assay for the detection of hemoglobin C[α2β26(A3)Glu → Lys] in cord blood using a monoclonal antibody We have generated a murine hybridoma that secretes a monoclonal antibody (mAb) that is highly specific for hemoglobin C (HbC) [226(A3)GluLys...
a good linear correlation was found between the BAM assay and a procedure usingl-leucine as substrate andd-2-hydroxyisocaproate dehydrogenase as a coupling enzyme (see above).12As already noted, the sensitivity of the spectrophotometric assay methods for detection of BCAA-AT activity is better th...