and helix–membrane interactions for membrane proteins in more general terms. Due to the presence of a central proline residue within the potentially membrane-buried part of the amino acid sequence, alamethicin resembles a transmembrane domain in a typical transport/channel protein with several bilayer...
More importantly, we find that those changes are not induced by Nedd8(Q40E) conjugation, indicating that the subtle change of a single Nedd8 amino acid is sufficient to revert the structure of the CRL catalytic core back to its unmodified form. Our results provide new insights into how ...
In addition, indirect fluorescence detection using salicylic acid for anionic charge peptides (basic buffers) or quinine for the positive mode (acidic buffer) have been applied. To accomplish lower detection limits for a broader range of species derivatization techniques have to be applied and all ...
and requires that each tRNA is paired with the correct amino acid to allow that codon-anticodon pairing results in proper protein formation2. In the mitochondria, this process is mediated by the mitochondrial
These enzymes must charge (aminoacylate with their corresponding amino acid) only their cognate tRNAs, discriminating against the myriad of very similar noncognate tRNAs, with the high fidelity necessary to ensure accurate translation of the genetic code. From examination of these interactions as ...
These enzymes must charge (aminoacylate with their corresponding amino acid) only their cognate tRNAs, discriminating against the myriad of very similar noncognate tRNAs, with the high fidelity necessary to ensure accurate translation of the genetic code. From examination of these interactions as ...
In this study, starting from the identification of a novel GPIbα p.Arg127Gln variant in LRR5 in a patient with a mild PT-VWD clinical and laboratory phenotype, we show for the first time that amino acid substitutions in the LRR may cause conformational ...
In contrast, the Seoul II mutation causes an amino acid substitution (AαGln328Pro) but does not form a stop codon. In characterizing heterozygous Seoul II fibrinogen, 2 findings were of particular interest. First, polymerization curves for the clotting of purified heterozygous Seoul II fibrinogen...
These observations raise an intriguing question as to how the subtle change of a single Nedd8 amino acid is able to negate the effect of neddylation in remodelling the ∼100-kDa CRL catalytic core. In the structure of the neddylated Cul5CTD–Rbx1 complex, Gln40 of Nedd8 is close to ...
Notably, the Arg99Gln amino acid substitution was reported in a subject presenting with a more complex and unique clinical phenotype characterized by distinctive facial features, DD/ID, cochlear aplasia, and bilateral colobomatous microphthalmia, suggesting the possible occurrence of phenotypic ...